国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
9期
848-849,851
,共3页
凝胶类%血型抗原%血型鉴定和交叉配血%抗体
凝膠類%血型抗原%血型鑒定和交扠配血%抗體
응효류%혈형항원%혈형감정화교차배혈%항체
Gels%Blood group antigens%Blood grouping and crossmatching%Antibodies
目的 探讨微柱凝胶技术在血型鉴定、交叉配血、抗体效价测定等相关输血试验中的应用,并对其进行方法学评价.方法 对微柱凝胶技术测定的55例Rh D阴性患者中的15例抗-Rh D呈阳性血样及21例正、反定型不符血样、29例交叉配血不合血样综合分析,并与试管抗人球蛋白结果对比.同时,对微柱凝胶技术检测的853例O型孕妇IgG型抗-A或抗-B效价结果进行分析.结果 抗-Rh D的检出率显示,微柱凝胶技术为27.27%(15/55),试管法为21.82%(12/55),效价在1:4以上阳性标本的检出两种方法一致.21例正、反定型不符的标本中,抗原或抗体减弱的11例用微柱凝胶技术检测,显示出反应的不一致性;而用试管法则呈现弱阳性反应.交叉配血中,微柱凝胶技术检出的29例阳性用试管法证实,2例为假阳性.O型孕妇血型效价测定以1:128为界,配偶为A型时,检出率为33.18%(144/434);配偶为B型时,检出率为33.17%(139/419),两者无显著差异.结论 微柱凝胶技术有操作简便、快速、结果客观的优点,但应重视其假阳性和假阴性.出现异常结果时,应将传统的试管法结合显微镜镜检.
目的 探討微柱凝膠技術在血型鑒定、交扠配血、抗體效價測定等相關輸血試驗中的應用,併對其進行方法學評價.方法 對微柱凝膠技術測定的55例Rh D陰性患者中的15例抗-Rh D呈暘性血樣及21例正、反定型不符血樣、29例交扠配血不閤血樣綜閤分析,併與試管抗人毬蛋白結果對比.同時,對微柱凝膠技術檢測的853例O型孕婦IgG型抗-A或抗-B效價結果進行分析.結果 抗-Rh D的檢齣率顯示,微柱凝膠技術為27.27%(15/55),試管法為21.82%(12/55),效價在1:4以上暘性標本的檢齣兩種方法一緻.21例正、反定型不符的標本中,抗原或抗體減弱的11例用微柱凝膠技術檢測,顯示齣反應的不一緻性;而用試管法則呈現弱暘性反應.交扠配血中,微柱凝膠技術檢齣的29例暘性用試管法證實,2例為假暘性.O型孕婦血型效價測定以1:128為界,配偶為A型時,檢齣率為33.18%(144/434);配偶為B型時,檢齣率為33.17%(139/419),兩者無顯著差異.結論 微柱凝膠技術有操作簡便、快速、結果客觀的優點,但應重視其假暘性和假陰性.齣現異常結果時,應將傳統的試管法結閤顯微鏡鏡檢.
목적 탐토미주응효기술재혈형감정、교차배혈、항체효개측정등상관수혈시험중적응용,병대기진행방법학평개.방법 대미주응효기술측정적55례Rh D음성환자중적15례항-Rh D정양성혈양급21례정、반정형불부혈양、29례교차배혈불합혈양종합분석,병여시관항인구단백결과대비.동시,대미주응효기술검측적853례O형잉부IgG형항-A혹항-B효개결과진행분석.결과 항-Rh D적검출솔현시,미주응효기술위27.27%(15/55),시관법위21.82%(12/55),효개재1:4이상양성표본적검출량충방법일치.21례정、반정형불부적표본중,항원혹항체감약적11례용미주응효기술검측,현시출반응적불일치성;이용시관법칙정현약양성반응.교차배혈중,미주응효기술검출적29례양성용시관법증실,2례위가양성.O형잉부혈형효개측정이1:128위계,배우위A형시,검출솔위33.18%(144/434);배우위B형시,검출솔위33.17%(139/419),량자무현저차이.결론 미주응효기술유조작간편、쾌속、결과객관적우점,단응중시기가양성화가음성.출현이상결과시,응장전통적시관법결합현미경경검.
Objective To explore the applicable value of microcolumn gel immunoassay(MGIA) in transfusion-related experiments(blood typing, cross matching, antibody titration), and carry out its methodical evaluation. Methods Fifteen anti-Rh D positive samples from 55 Rh D negative patients, twenty-one cases of discrepant blood typing and twenty-nine cross matching positive samples were studied by applying MGIA and routine tube test simultaneously. Antibody titer data from 853 pregnant women with type O were brought into statistics. Results In 55 Rh D negative samples, the positive rate of Rh D antibody tested by MGIA and tube test was 27.27%(15/55) and 21.82 % (12/55) respec-tively,and the samples with titer 1:4 above got the coincident results by the two methods. For twen-ty-one discrepant blood typing samples, 11 cases with weak reaction showed discrepant results by MGIA;however, all samples showed weak reaction by tube test. In 29 samples with cross matching positive by MGIA,2 cases were confirmed to be negative by tube test. For the pregnant women with type O,IgG antibody titer abnormal ratio was 33.18%(144/434) if their respective husbands was with type A,33.17%(139/419) if their respective husbands was with type B.Conclusion MGIA is charac-teristic of convenience, fastness and reliability of test results, but its false-positive or false-negative should be paid attention to. When abnormal results occur, traditional tube test as well asmicroscopic examination should be performed in parallel.
