CAJ | 학술논문

目的:观察电针治疗白细胞减少症的疗效、特点及其与脾脏超微结构改变的关系,探讨针灸调节白细胞的作用机制.方法:通过家兔静脉注射环磷酰胺复制白细胞减少症模型,采用电针治疗,分别每天于耳缘静脉采血进行外周血白细胞计数和分类,观察血象变化,并于处死前麻醉动物,开腹取脾组织电镜下观测脾血窦基膜小孔口径大小.结果:与模型对照组比较,电针治疗组动物外周血白细胞数量迅速上升,粒细胞发生核右移(P＜0.01).电针治疗组脾血窦基膜小孔口径大于模型对照组(P＜0.05).结论:电针可以使白细胞减少的机体白细胞数量迅速得到恢复,在治疗的早期主要与调节脾脏功能有关.
목적:관찰전침치료백세포감소증적료효、특점급기여비장초미결구개변적관계,탐토침구조절백세포적작용궤제.방법:통과가토정맥주사배린선알복제백세포감소증모형,채용전침치료,분별매천우이연정맥채혈진행외주혈백세포계수화분류,관찰혈상변화,병우처사전마취동물,개복취비조직전경하관측비혈두기막소공구경대소.결과:여모형대조조비교,전침치료조동물외주혈백세포수량신속상승,립세포발생핵우이(P＜0.01).전침치료조비혈두기막소공구경대우모형대조조(P＜0.05).결론:전침가이사백세포감소적궤체백세포수량신속득도회복,재치료적조기주요여조절비장공능유관.
Objective: To explore the underlying mechanism by which acupuncture regulates the peripheral leukocyte count and observe the relationship between the effects of electroacupuncture (EA) and the spleen ultrastructure in leukopenia. Methods: Leukopenia models of rabbits were established by injecting cyclophosphamide (CY) into rabbits' ear vein,and then the rabbits were treated with acupuncture. The peripheral leukocyte count and classification were measured daily. At last, after the animals were anesthesized, the abdominal cavity was opened, and a piece of spleen tissue was cut. The diameter of splenic sinusoid basal lamina eyehole was measured under electric microscope. Results: The peripheral blood leukocyte count in the EA group increased significantly with shift to right of the granulocyte nuclei compared with model control group (P＜0.01). Moreover, the calibers of splenic sinusoid basal lamina eyehole in the EA group were larger than those in the model control group (P＜0.05). Conclusion: Acupuncture can enhance the peripheral leukocyte count by promoting spleen activity in the early phase of leukopenia.