CAJ | 학술논문

目的探讨用表面增强基质辅助激光接下来离子化-飞行时间质谱(SELDI-TOF-MS)筛选视网膜母细胞瘤(retinoblastoma,Rb)血清标志蛋白,为Rb筛选、早期诊断及鉴别诊断寻找新的方法.方法采用IMAC30和CM10两种蛋白芯片对18例Rb患者和17名年龄匹配的正常小儿雪清进行了蛋白质谱检测.统计分析采用Ciphergenproteinchip3.0.2.软件,两组间蛋白峰强度的比较采用t检验,率的比较采用 Fisher 精确概率法.结果 IMAC30蛋白芯片结果显示:与正常幼儿相比,视网膜母细胞瘤患者血清中有21个,质/荷比(m/z) 分别为:7746, 7014, 11713, 3049, 7084, 7299, 5888, 2544, 12575, 5489, 9658, 9575, 9929, 10161, 8955, 1886, 10617, 6209,2411,7374,和6614;低表达有5个,分别为8382,7923,7972,8590和66576,统计学检验p<0.01.其中以m/z7014峰进行两组统计学分析时,敏感性和特异性分别为94.4%和82.4%.与正常小儿相比,经CM10蛋白芯片分析显示:视网膜母细胞瘤患者血清中有4个差异表达的蛋白峰,其中高表达有3个,质/荷比(m/z )分别为5888,6097和7798;低表达蛋白锋为m/z8590.统计学检验p<0.01.当选择m/z7798峰进行统计学分析时,敏感性和特异性分别是83.3%和70.6%.结论视网膜母细胞瘤患者血清中存在较多标志蛋白,SELDL-TOF-MS蛋白芯片技术有望成为Rb筛查、早期诊断及鉴别诊断的新的有效工具.
목적 탐토용표면증강기질보조격광접하래리자화-비행시간질보(SELDI-TOF-MS)사선시망막모세포류(retinoblastoma,Rb)혈청표지단백,위Rb사선、조기진단급감별진단심조신적방법.방법 채용IMAC30화CM10량충단백심편대18례Rb환자화17명년령필배적정상소인설청진행료단백질보검측.통계분석채용Ciphergenproteinchip3.0.2.연건,량조간단백봉강도적비교채용t검험,솔적비교채용 Fisher 정학개솔법.결과 IMAC30단백심편결과현시:여정상유인상비,시망막모세포류환자혈청중유21개,질/하비(m/z) 분별위:7746, 7014, 11713, 3049, 7084, 7299, 5888, 2544, 12575, 5489, 9658, 9575, 9929, 10161, 8955, 1886, 10617, 6209,2411,7374,화6614;저표체유5개,분별위8382,7923,7972,8590화66576,통계학검험p<0.01.기중이m/z7014봉진행량조통계학분석시,민감성화특이성분별위94.4%화82.4%.여정상소인상비,경CM10단백심편분석현시:시망막모세포류환자혈청중유4개차이표체적단백봉,기중고표체유3개,질/하비(m/z )분별위5888,6097화7798;저표체단백봉위m/z8590.통계학검험p<0.01.당선택m/z7798봉진행통계학분석시,민감성화특이성분별시83.3%화70.6%.결론시망막모세포류환자혈청중존재교다표지단백,SELDL-TOF-MS단백심편기술유망성위Rb사사、조기진단급감별진단적신적유효공구.
AIM:To find new biomarkers in the sera of retinoblastoma (Rb) patients with surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI TOF MS) and protein chip technique.METHODS:SELDI TOF MS, IMAC30 and CM10 protein chips were used to analyze the protein profiles from sera of 18 patients with Rb and 17 age matched controls. The protein profiling was analyzed statistically by Ciphergen protein chip software 3.0.2. The test was applied to compare the protein peak intensity. Fisher's exact test was used to compare the predominance of differential protein peaks appeared in patients.RESULTS:With IMAC30 protein chips, there were 26 proteins which appeared different in sera of patients with Rb compared to normal children. Among them, 21 proteins, I.e. 7746, 7014, 11713, 3049, 7084, 7299, 5888, 2544, 12575, 5489, 9658, 9575, 9929, 10161, 8955, 1886, 10617, 6209, 2411, 7374, 6614m/z were up regulated and 5 proteins, I.e. 8382, 7923, 7972, 8590, 66576m/z, were down regulated(P<0.01). Using the 7014 protein peak for statistical analysis, we could differentiate the patients with Rb from the healthy children with a sensitivity of 94.4% and a specificity of 82.4%. By CM10 protein chips, 4 proteins, including 3 up regulated proteins(5888, 6097, 7798 and 1 down regulated protein (8590m/z), were detected in Rb patients (P<0.01). The sensitivity and specificity were 83.3% and 70.6% respectively when 7798m/z protein peak was selected for statistical analysis.CONCLUSION:There are a few candidates as Rb biomarkers in the sera of Rb patients. SELDI TOF MS protein chip technology could be a potential method in the clinical screening test of Rb.