CAJ | 학술논문

报道了一种可控的通过DNA复合物在微流路中杂交固定蛋白质的方法.微流路系统中的玻璃基底上固定寡聚核苷酸,其中的层流提供了不同的DNA-蛋白质复合物.DNA的特异性识别可以将蛋白通过表面寻址固定在基底上.并且在体系中引入了全内反射荧光技术来追踪整个过程.此方法的特异性和灵敏度均较高,且蛋白质的固定和去除可重复.实验结果显示,同时检测特异性和非特异性的识别,可以有效提高生物检测的准确性.这项技术可以提高具有微流路结构的生物传感器装置的检测质量.
보도료일충가공적통과DNA복합물재미류로중잡교고정단백질적방법.미류로계통중적파리기저상고정과취핵감산,기중적층류제공료불동적DNA-단백질복합물.DNA적특이성식별가이장단백통과표면심지고정재기저상.병차재체계중인입료전내반사형광기술래추종정개과정.차방법적특이성화령민도균교고,차단백질적고정화거제가중복.실험결과현시,동시검측특이성화비특이성적식별,가이유효제고생물검측적준학성.저항기술가이제고구유미류로결구적생물전감기장치적검측질량.
The feasibility of controlled protein immobilization via DNA conjugation by utilizing laminar flow in a microfluidic device was demonstrated. The glass surface in a microchannel was treated by oligonucleotides. The laminar flow brought different protein-DNA conjugates parallel into the microchannel. DNA recognition allows proteins to be delivered to the desired location. The total internal reflection fluorescence was also applied to monitor the process. Both the specificity and sensitivity were high, and the immobilization and removal of the proteins were repeatable. It was shown that with parallel detection of specific and non-specific recognitions, the accuracy of bioassay would be effectively enhanced. This strategy could improve the performing quality of biosensors in microfluidic devices.