CAJ | 학술논문

目的通过对大鼠实验前给予左旋-硝基精氨酸甲脂(L-NAME,NO前体左旋精氨酸类似物)后,观察急性高眼压后大鼠视网膜电图(ERG)b波幅的变化,iNOSmRNA的表达情况.探讨L-NAME和iNOS在高眼压视网膜损伤中的作用.方法 Wister大鼠48只随机分为6组,分别为高眼压30 min、90 min组、高眼压后12 h组、注射L-NAME+高眼压30 min、90 min组和注射L-NAME+高眼压后12h组.前房加压灌注成高眼压模型.Ahplaldamk15型视电生理仪检测FERG-b波波幅变化.RT-PCR法检测iNOSmRNA的表达.结果 L-NAME组的ERG-b波波幅与对照组相比前者明显恢复(P＜0.01).高眼压30 min,90 min,12 hiNOSmRNA的表达逐渐增强,但L-NAME组中iNOSmRNA的表达明显低于对应的高眼压组(P＜0.01).结论 L-NAME做为一氧化氮合酶的抑制剂,通过抑制iNOS合成具有细胞毒性的NO,有助于缺血后的视网膜功能的恢复,对视网膜起到保护作用.
목적 통과대대서실험전급여좌선-초기정안산갑지(L-NAME,NO전체좌선정안산유사물)후,관찰급성고안압후대서시망막전도(ERG)b파폭적변화,iNOSmRNA적표체정황.탐토L-NAME화iNOS재고안압시망막손상중적작용.방법 Wister대서48지수궤분위6조,분별위고안압30 min、90 min조、고안압후12 h조、주사L-NAME+고안압30 min、90 min조화주사L-NAME+고안압후12h조.전방가압관주성고안압모형.Ahplaldamk15형시전생리의검측FERG-b파파폭변화.RT-PCR법검측iNOSmRNA적표체.결과 L-NAME조적ERG-b파파폭여대조조상비전자명현회복(P＜0.01).고안압30 min,90 min,12 hiNOSmRNA적표체축점증강,단L-NAME조중iNOSmRNA적표체명현저우대응적고안압조(P＜0.01).결론 L-NAME주위일양화담합매적억제제,통과억제iNOS합성구유세포독성적NO,유조우결혈후적시망막공능적회복,대시망막기도보호작용.
[Objective] To investigate the effect of L-NAME and induced nitric oxide synthase (iN0S) in rat retinal damage under hypertension. [Methods] 48 Wistar rats were divided into six groups randomly comprising:ocular hypertension 30 min, 90 min, 12h after reperfusion, injection L-NAME+ hypertension 30 min, injection L-NAME+ hypertension 90 min, and injection L-NAME+12 h after reperfusion, seperately. Elevation of the ocular pressure in the anterior chamber of the rat eye caused retina ischemic damage. Then b-wave of ERGs and expression of iNOS mRNA were recorded by RT-PCR. [Results] Pretreatment with intravenous L-NAME greatly enhanced b-wave amplitude (P ＜0.01) compared with the groups of hypertension. The expression of iNOS mRNA was lower than that of untreated groups. [Conclusion] As an inhibitor of iNOS, L-NAME may play an important role in protecting retina from ischemic and post-ischemic injury.