CAJ | 학술논문

目的:研究扇贝裙边糖氨聚糖对氧化低密度脂蛋白(ox-LDL)诱导的U937细胞泡沫化过程中血管内皮生长因子(VEGF)的影响,探讨其抗动脉粥样硬化作用的机理.方法:采用U937细胞与80mg/L的ox-LDL孵育48h建立U937泡沫细胞模型.将培养的U937细胞随机分为六组,正常对照组、模型组(ox-LDL)、肝素对照组(ox-LDL加100mg/L肝素)和低、中、高浓度的SS-GAG药物组(ox-LDL加200mg/L,400mg/L,800mg/L的SS-GAG).采用酶联免疫吸附实验(ELISA)检测细胞分泌的VEGF的量,观察不同浓度SS-GAG对U937细胞泡沫化过程中VEGF表达量的影响.结果:培养的U937泡沫细胞中VEGF的表达量明显高于正常U937细胞(P＜0.01),而加入SS-GAG的药物组和肝素对照组则有不同程度降低,以800mg/mL药物组降低最为明显(P＜0.01).结论:泡沫细胞形成过程中伴有VEGF的高表达,SS-GAG能够抑制其表达从而发挥抗动脉粥样硬化作用.
목적:연구선패군변당안취당대양화저밀도지단백(ox-LDL)유도적U937세포포말화과정중혈관내피생장인자(VEGF)적영향,탐토기항동맥죽양경화작용적궤리.방법:채용U937세포여80mg/L적ox-LDL부육48h건립U937포말세포모형.장배양적U937세포수궤분위륙조,정상대조조、모형조(ox-LDL)、간소대조조(ox-LDL가100mg/L간소)화저、중、고농도적SS-GAG약물조(ox-LDL가200mg/L,400mg/L,800mg/L적SS-GAG).채용매련면역흡부실험(ELISA)검측세포분비적VEGF적량,관찰불동농도SS-GAG대U937세포포말화과정중VEGF표체량적영향.결과:배양적U937포말세포중VEGF적표체량명현고우정상U937세포(P＜0.01),이가입SS-GAG적약물조화간소대조조칙유불동정도강저,이800mg/mL약물조강저최위명현(P＜0.01).결론:포말세포형성과정중반유VEGF적고표체,SS-GAG능구억제기표체종이발휘항동맥죽양경화작용.
Objective: To study the effects of glycosaminoglycan from scallop skirt (SS-GAG) on the expression of vascular endothelial growth factor (VEGF) and the mechanism of anti-atherosclerosis action of SS-GAG. Methods: U937 cells were incubated with 80mg/L oxidized low density lipoprotein (ox-LDL) for 48h to establish a macrophage-derived foam cell model. In addition, U937 cells were divided into 6 groups: ①control group; ②ox-LDL group; ③ox-LDL+200mg/L SS-GAG group; ④ox-LDL+400 mg/L SS-GAG group; ⑤ox-LDL+800 mg/L SS-GAG group; ⑥ox-LDL +Heparin 100 mg/L group.After 48h's incubation, the concentration of VEGF in the medium was determined by ELISA. Results: The expression of VEGF in U937 foam cells was obviously higher than that of the control group. After treatment with heparin (100 mg/L) and SS-GAG of different concentrations (200mg/L, 400 mg/L, 800 mg/L), the expression of VEGF decreased obviously, especially in the ox-LDL+800 mg/L SS-GAG group (P＜0.01). Conclusion: The antiatherogenic effect of SS-GAG is probably due to its ability to inhibit VEGF expression.