CAJ | 학술논문

目的检测与支气管哮喘遗传相关的基因。方法将门诊或病房确诊为哮喘的病人按实验要求分为两组，一组为有明显家族遗传史的患者，另一组为没有家族史的散发患者。首先从实验对象的外周血中提取DNA，PCR扩增IL-4的第二个内含子中的一段TG重复序列，然后用8％的聚丙烯酰胺凝胶电泳检测这段基因的多态性。结果在这段基因序列中检测出4种多态性，其中等位基因表型1和等位基因表型2在有遗传史的哮喘患者与无遗传史的哮喘患者中出现的频率有区别。结论IL-4的第二个内含子中的TG重复序列的多态性可能与哮喘的遗传有关。
목적검측여지기관효천유전상관적기인。방법장문진혹병방학진위효천적병인안실험요구분위량조，일조위유명현가족유전사적환자，령일조위몰유가족사적산발환자。수선종실험대상적외주혈중제취DNA，PCR확증IL-4적제이개내함자중적일단TG중복서렬，연후용8％적취병희선알응효전영검측저단기인적다태성。결과재저단기인서렬중검측출4충다태성，기중등위기인표형1화등위기인표형2재유유전사적효천환자여무유전사적효천환자중출현적빈솔유구별。결론IL-4적제이개내함자중적TG중복서렬적다태성가능여효천적유전유관。
Objective to detect polymorphism of asthma related genes. Methods Patients diagnosed asthma were divided into twogroups , one group has genetic susceptibility , the other has no such character. DNA were extracted from the peripheral blood, then TG se-quence in second intron of IL-4 was amplified by PCR, the polymorphism of that segment were then detected through 8% po]yacrylamidegel. Result four kinds of penotype were found in such segment. The frequency of phenotype 1 and type 2 of in test group are statisticallydifferent from that in the check group. But the other two phenotype frequencies are just the same in two groups. Conclusion the differentfrequency of phenotype I and phenotype 2 in two groups may give us some meaning in asthma genetics.