CAJ | 학술논문

采用正交设计研究了不同植物激素配比、蔗糖质量浓度、外植体取材部位以及单宁含量等因素对山核桃组织培养中愈伤组织形成的影响。结果表明：WPM＋BA 8.0 mg*L－1＋IBA 2.0 mg*L－1＋GA 1.0 mg*L－1＋蔗糖30 mg*L－1是最适培养基；顶芽和第4芽段为较适宜的山核桃愈伤组织诱导的取材部位；不同芽段愈伤组织的形成率与其单宁含量不相关，而与芽段愈伤组织形成的部位有关，且随着单宁含量的减少，愈伤组织形成的部位向切口上移。选取1年生实生苗茎尖和茎段，配比合理的植物激素加以诱导，可提高山核桃嫁接成活率。表5参11
채용정교설계연구료불동식물격소배비、자당질량농도、외식체취재부위이급단저함량등인소대산핵도조직배양중유상조직형성적영향。결과표명：WPM＋BA 8.0 mg*L－1＋IBA 2.0 mg*L－1＋GA 1.0 mg*L－1＋자당30 mg*L－1시최괄배양기；정아화제4아단위교괄의적산핵도유상조직유도적취재부위；불동아단유상조직적형성솔여기단저함량불상관，이여아단유상조직형성적부위유관，차수착단저함량적감소，유상조직형성적부위향절구상이。선취1년생실생묘경첨화경단，배비합리적식물격소가이유도，가제고산핵도가접성활솔。표5삼11
Effects of such factors as ratios of different plant hormones，mass concentrations of surose，the part from which explants were obtained and the content of tannins on the formation of callus in the tissue culture of Carya cathayensis were studied by means of an orthogonal design．It's showed that WPM＋BA 8.0 mg*L－1＋IBA 2.0 mg*L－1＋GA 1.0 mg*L－1＋sucrose 30 mg*L－1 was an optimal medium；the portion between apical buds and the 4th bud was suitable to induce callus in Carya cathayensis；and the formation rate of callus for loci of the buds was not correlated with the content of tannins，but in correlation with the loci where callus was formed．In addition，the smaller the content of tannins，the farther the locus where callus formed was from the cut．The key of raising survival rate of grafting was the selection of scions and the proportions of hormones to be used in grafting．