CAJ | 학술논문

分别以葡萄糖氧化酶法、放射免疫分析法对服用微粒剂后的四氧嘧啶致糖尿病小鼠与狗的血糖、血清胰岛素进行测定，验证确有外源性胰岛素进入动物体内，在给药后1h动物血清胰岛素增至最高，约2h动物血糖降至最低，且作用强度、时间与血清胰岛素变化相对应。给药剂量在4.0～35.0u/kg范围，存在量效关系：Y(%)=13.21-1.707X(u/kg),r=0.9788。给小鼠腹腔注射葡萄糖(2g/kg)可见，微粒剂(17u/kg)能对抗并减缓因注射外源还原性单糖引起的动物血糖升高，0.5h可抵抗百分率达86%(P<0.005)。经四氧嘧啶糖尿病大鼠胃肠道不同部位给药发现：给药部位不同，微粒剂降血糖程度有显著差别，其中经回肠部位给药效果最好，降血糖百分率达56%(P<0.01)。
분별이포도당양화매법、방사면역분석법대복용미립제후적사양밀정치당뇨병소서여구적혈당、혈청이도소진행측정，험증학유외원성이도소진입동물체내，재급약후1h동물혈청이도소증지최고，약2h동물혈당강지최저，차작용강도、시간여혈청이도소변화상대응。급약제량재4.0～35.0u/kg범위，존재량효관계：Y(%)=13.21-1.707X(u/kg),r=0.9788。급소서복강주사포도당(2g/kg)가견，미립제(17u/kg)능대항병감완인주사외원환원성단당인기적동물혈당승고，0.5h가저항백분솔체86%(P<0.005)。경사양밀정당뇨병대서위장도불동부위급약발현：급약부위불동，미립제강혈당정도유현저차별，기중경회장부위급약효과최호，강혈당백분솔체56%(P<0.01)。
The levels of glucose and insulin (INS) in serum were detected bythe methods of glucose oxidation enzyme and radioimmunoassay for the aloxan-caused diabetic mice and normal dogs to which INS-MP was adminstrated. It was indicated that there was exogenous INS in above animals. The highest INS level was at 1 h after oral administering INS-MP, and at 2 h for the lowest point of glucose. The action intensity and the time corresponded to the change of serum INS. There was the correlation between hypoglycemic percentage and dosage, Y(%)=13.21-1.707X(u/kg) in the dose range from 4.0 to 35.0. The INS-MP could retard the increase of mice′ blood glucose caused by exogenous glucose, and the anti-glucose percentage was 86% (P<0.005). It was showed through administering the INS-MP to different sites in diabetic rats′ gastrointestinal tracts that the best absorption site was the ileum and the hypoglycemic strength was 56% (P<0.01).