CAJ | 학술논문

目的利用肿瘤患者自体血清筛选患者肿瘤cDNA表达文库(SEREX)，寻找肿瘤特异性抗原是肿瘤免疫学研究的新策略，由此方法所获B细胞抗原肽可为进一步寻找T细胞抗原肽，开展肿瘤免疫治疗和诊断提供线索。方法采用SEREX方法，筛选中国人卵巢癌cDNA表达文库。对获得的阳性克隆片段测序鉴定和进行BLAST同源性比较。采用RT-PCR，分析正常组织和肿瘤组织及肿瘤细胞株中6个新的EST片段的表达。结果所获基因片段大多数为已知抗原基因(20/27)。根据其来源又可归类为结构基因、线粒体基因和调控基因。另外，获得6个未知的EST片段。RT-PCR的结果表明，4个EST(EST8788、1750、1754和3533)片段在肿瘤和正常组织中均有表达，2个EST在正常组织中未见表达，而在肿瘤组织中有表达，可能是与肿瘤相关或特异的EST片段。结论SEREX方法不失为一种有效筛选卵巢癌肿瘤抗原的新方法，所获肿瘤抗原候选基因，可进一步进行结构和功能的研究。
목적이용종류환자자체혈청사선환자종류cDNA표체문고(SEREX)，심조종류특이성항원시종류면역학연구적신책략，유차방법소획B세포항원태가위진일보심조T세포항원태，개전종류면역치료화진단제공선색。방법채용SEREX방법，사선중국인란소암cDNA표체문고。대획득적양성극륭편단측서감정화진행BLAST동원성비교。채용RT-PCR，분석정상조직화종류조직급종류세포주중6개신적EST편단적표체。결과소획기인편단대다수위이지항원기인(20/27)。근거기래원우가귀류위결구기인、선립체기인화조공기인。령외，획득6개미지적EST편단。RT-PCR적결과표명，4개EST(EST8788、1750、1754화3533)편단재종류화정상조직중균유표체，2개EST재정상조직중미견표체，이재종류조직중유표체，가능시여종류상관혹특이적EST편단。결론SEREX방법불실위일충유효사선란소암종류항원적신방법，소획종류항원후선기인，가진일보진행결구화공능적연구。
Aim Serological identification of antigens by recombinantexpression cloning (SEREX)search for the B-cell-defined tumor specific antigen and peptide. It would provide new approaches for identification of tumor specific antigens, which would give light to immunological diagnosis and therapy of ovarian cancer in clinics. Methods We screened a cDNA expression library of ovarian cancer with autologous serum and determined the expression patterns of news ESTs in normal tissues, carcinoma tissues and various kinds of tumor cell lines by RT-PCR. Results Of all the antigens we obtained, most are encoded by the identified genes, which were expressed in cytoplasm or nuclei. Others are saml ESTs (expression sequence tags), which were encoded by unknown genes. RT-PCR results showed that two ESTs could be considered as the candidate genes for the tumor-associated or -specific antigens. Further study will focus on the structure and function analysis of these two EST fragments. Conclusion SEREX is an effective method for searching new tumor antigens,which can be further studied on their sturctures and functions.