吉林农业大学学报
吉林農業大學學報
길임농업대학학보
JOURNAL OF JILIN AGRICUL TURAL UNIVERSITY
2001年
2期
35-37,40
,共4页
魏松红%王罡%张领兵%张艳贞%杨家书
魏鬆紅%王罡%張領兵%張豔貞%楊傢書
위송홍%왕강%장령병%장염정%양가서
小麦白粉病菌%生理小种%鉴定%RAPD分析
小麥白粉病菌%生理小種%鑒定%RAPD分析
소맥백분병균%생리소충%감정%RAPD분석
对1999~2000年小麦白粉病菌的生理小种进行了鉴定,并分析了小种种群的变化。应用23个随机引物对白粉病菌8个不同生理小种进行RAPD分析,其中11个引物的扩增带型在不同生理小种间出现差异,共扩增出59条谱带,多态性为47.5%。通过对RAPD分析结果进行聚类分析,初步探明了白粉菌小种间的亲缘关系及其与小种毒力高低的相关性。
對1999~2000年小麥白粉病菌的生理小種進行瞭鑒定,併分析瞭小種種群的變化。應用23箇隨機引物對白粉病菌8箇不同生理小種進行RAPD分析,其中11箇引物的擴增帶型在不同生理小種間齣現差異,共擴增齣59條譜帶,多態性為47.5%。通過對RAPD分析結果進行聚類分析,初步探明瞭白粉菌小種間的親緣關繫及其與小種毒力高低的相關性。
대1999~2000년소맥백분병균적생리소충진행료감정,병분석료소충충군적변화。응용23개수궤인물대백분병균8개불동생리소충진행RAPD분석,기중11개인물적확증대형재불동생리소충간출현차이,공확증출59조보대,다태성위47.5%。통과대RAPD분석결과진행취류분석,초보탐명료백분균소충간적친연관계급기여소충독력고저적상관성。
The physiological strains of Erysiphe graminis were determined in 1999~2000 and the changes of strain groups were analyzed. Differences were found among 8 strains from RAPD analysis. Out of 23 random primers, 11 were polymorphic. Altogether 59 RAPD bands were found and the polymorphic rate was 47.5%. Genetic relationships among the 8 strains and their correlation to the virulence of the strains were studied through cluster analysis on the RAPD bands.