CAJ | 학술논문

用基因枪转基因技术将高抗白叶枯病的Xa21基因导入中国三系杂交稻恢复系(明恢63)和保持系(皖B)中，获得转基因水稻系，其中4个株系只含有Xa21基因，不含选择标记潮霉素抗性基因hph。筛选抗白叶枯病转基因纯合系，在田间种植6代，用PCR检测证明，Xa21基因能稳定遗传表达。用转基因水稻配制两系杂交稻，杂交组合F1含有Xa21基因，既抗白叶枯病，又有较大的增产潜力。
용기인창전기인기술장고항백협고병적Xa21기인도입중국삼계잡교도회복계(명회63)화보지계(환B)중，획득전기인수도계，기중4개주계지함유Xa21기인，불함선택표기조매소항성기인hph。사선항백협고병전기인순합계，재전간충식6대，용PCR검측증명，Xa21기인능은정유전표체。용전기인수도배제량계잡교도，잡교조합F1함유Xa21기인，기항백협고병，우유교대적증산잠력。
Xa21 gene and hph gene were co-transferred into Chinese 3-linehybrid rice lines Minghui 63(restorer line) and Wan B (maintainence line )by biolistics gene transfer method. A total of 5 transgenic rice lines with enhanced resistance against rice bacterial blight (BB) were obtained. Four of the transgenic lines were found to have only Xa21 gene integrated into the genome,and the other one have both Xa21 and hph integrated. Molecular analysis to the transgenic plants (up to six generations) showed that Xa21 gene had been integrated into the plant genome. The offsprings inherited the resistance from Xa21 stable. The Xa21 transgenic rice plants have been used as the restorer line for 2-line hybrid combinations. F1 of some combinations were found to show strong BB resistance and high yield-increasing potentiality.