CAJ | 학술논문

目的探索BALB/c和C57BL/6两个品系在有关实验中的不同作用。方法选取了结合随机测序与生物信息学分析设计合成的神经系统表达的一些基因的反义核酸(antisense)中的2个，用Hamilton微量注射器将其分别定量注射到BALB/c和C57BL/6小鼠的侧脑室，并分别设注射生理盐水和随机序列核酸(Scramble)的对照组。每一反义核酸实验组和对照组各注射10只小鼠，之后观察实验组与对照组在不同行为学实验中的差异。小鼠的行为学检测模型为：考察日常代谢能力的摄食量，考察Locomotionactivity(移动)的旷场行为，考察疼痛阈值的甩尾试验和考察记忆能力的步下法实验。结果注射No.1基因的反义核酸后，两品系的实验组均在测试记忆力的步下法(Step-downTest)试验中表现出记忆力减弱，且与对照组差异明显，说明No.1基因的功能确与记忆相关。注射No.2基因的反义核酸后，在测试移动能力的旷场行为(OpenFieldBehavior)试验中，BALB/c实验组跨格、直立行为均比对照组明显减少，说明受此反义核酸影响显著，而C57BL/6实验组则与对照组无大的差异。此外，在生理盐水对照组和随机序列核酸对照组的实验中以及其他行为学模型的实验中，两品系也存在着一定的差异。结论用遗传背景不同的多品系进行相关实验，可进一步建立新基因功能初筛中有显著结果的基因的复筛平台；同时，实验结果对于进一步研究什么样的品系适用于什么样的实验将具有较大的意义。
목적 탐색BALB/c화C57BL/6량개품계재유관실험중적불동작용。방법 선취료결합수궤측서여생물신식학분석설계합성적신경계통표체적일사기인적반의핵산(antisense)중적2개，용Hamilton미량주사기장기분별정량주사도BALB/c화C57BL/6소서적측뇌실，병분별설주사생리염수화수궤서렬핵산(Scramble)적대조조。매일반의핵산실험조화대조조각주사10지소서，지후관찰실험조여대조조재불동행위학실험중적차이。소서적행위학검측모형위：고찰일상대사능력적섭식량，고찰Locomotionactivity(이동)적광장행위，고찰동통역치적솔미시험화고찰기억능력적보하법실험。결과 주사No.1기인적반의핵산후，량품계적실험조균재측시기억력적보하법(Step-downTest)시험중표현출기억력감약，차여대조조차이명현，설명No.1기인적공능학여기억상관。주사No.2기인적반의핵산후，재측시이동능력적광장행위(OpenFieldBehavior)시험중，BALB/c실험조과격、직립행위균비대조조명현감소，설명수차반의핵산영향현저，이C57BL/6실험조칙여대조조무대적차이。차외，재생리염수대조조화수궤서렬핵산대조조적실험중이급기타행위학모형적실험중，량품계야존재착일정적차이。결론 용유전배경불동적다품계진행상관실험，가진일보건립신기인공능초사중유현저결과적기인적복사평태；동시，실험결과대우진일보연구십요양적품계괄용우십요양적실험장구유교대적의의。
Objective To study the differe nt usage of BALB/c and C57BL/6，thetwo strains of laboratory mouse with d i fferent genetic backgrounds，in relevant experiments.Methods Authors chose antisense oligo-nucl eotides of two genes expressed in central nervous system by random sequencing an d bioinformatics analysis， injected them into the lateral ventricles of mou se brain using Hamilton micro-syringe respectively. There are also two control groups of saline and scramble. Each group has ten mice. And then several behav ioral tests were carried out. They are Feeding for metabolic ability，Open Fiel d Behavior for locomotion activity，Tail Flick Latency(TFL)for limen of pain，Ste p-down Test for memory.Results It was found that after the injection of No.1 antis ense both two strains of mice showed damaged memory and there were obvious diffe r ences between experimental groups and control groups.It is said that the functi on of No.1 gene is correlative with memor y. After the No.2 antisense was injected，BALB/c strain experimental group sho wed obvious decrease in numbers of step-across and standing-up in open field behavi or test compared with control groups. But in C57BL/6 mice，there we ren't ob vious differences between experimental group and control groups. And there were also differences between the control groups of the two strains. Conclusion The results show that using laboratory mouse st rains with different genetic backgrounds can set up a further monitoring system for the screening of novel gene funtion. Simultaneously the results have certai n significance in finding which kinds of strains are suitable for which kinds of experiments.