CAJ | 학술논문

为了从分子水平上检验野生棉遗传特性向陆地棉转育的结果以及育成种质之间的遗传差异，通过等电聚焦电泳和RAPD技术，对来自科遗181陆地棉品系与野生比克氏棉杂交后代的6个遗传稳定的不同种质系及其亲本的过氧化物酶同工酶图谱和DNA指纹图谱进行了分析。结果如下：（1） 6个种质系的基本酶谱特征均倾向于陆地棉亲本，但在其中2个种质系的过氧化物酶图谱中，观察到各具有1条等电点值（PI）为4.85的野生亲本的特征带；（2） DNA指纹图谱的分析表明，不同种质系之间在基因组水平上具有高度的异质性。并且在OPO10和OPO11 2个引物的扩增图谱中观察到4个育成种质系具有野生比克氏棉亲本的特征带。
위료종분자수평상검험야생면유전특성향륙지면전육적결과이급육성충질지간적유전차이，통과등전취초전영화RAPD기술，대래자과유181륙지면품계여야생비극씨면잡교후대적6개유전은정적불동충질계급기친본적과양화물매동공매도보화DNA지문도보진행료분석。결과여하：（1） 6개충질계적기본매보특정균경향우륙지면친본，단재기중2개충질계적과양화물매도보중，관찰도각구유1조등전점치（PI）위4.85적야생친본적특정대；（2） DNA지문도보적분석표명，불동충질계지간재기인조수평상구유고도적이질성。병차재OPO10화OPO11 2개인물적확증도보중관찰도4개육성충질계구유야생비극씨면친본적특정대。
To study the effectiveness of Gossypium interspecific hybrid breeding on molecular level, the six germplasm lines from interspecific hybrids between G. hirsutum and G. bickii were analysed by the techniques of RAPD and isozyme electrophoresis. The main results were summarized as follows: (1) The basic zymograms of peroxidase isozyme of the six germplasm lines were similar to their G. hirsutum parent, but two out of the germplasm lines showed one specific band of the wild parent of G. bickii (pI 4.85); (2) The RAPD fingerprint showed distinct difference of genomic DNA among the germplasm lines; (3) The four specific DNA fragments of G. bickii were observed in RAPD patters of four out of the six germplasm lines.