CAJ | 학술논문

枯草芽孢杆菌(Bacillus subtilis) SO113对水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae)有强烈的抑菌作用(表现出一透明抑菌圈)。于培养液上清中加入60%饱和度的硫酸铵沉淀所得的蛋白粗提液对热稳定，对链霉蛋白酶E不敏感，对蛋白酶K部分敏感，对中国各稻区白叶枯病菌的7种致病型都有强烈的抑杀作用。蛋白粗提液100℃加热15 min后脱盐，经DEAE-Sepharose Fast Flow柱层析得3个未分开的抗菌活性峰，未吸附部分经CM-Sepharose Fast Flow柱层析，得到1个主活性峰。由于B.subtilis SO113的分泌蛋白对水稻白叶枯病菌表现出良好的广谱抗性以及丰富的抗菌活性峰，因此对其进一步的研究和设法克隆编码抗菌蛋白的基因都具有重要意义。
고초아포간균(Bacillus subtilis) SO113대수도백협고병균(Xanthomonas oryzae pv.oryzae)유강렬적억균작용(표현출일투명억균권)。우배양액상청중가입60%포화도적류산안침정소득적단백조제액대열은정，대련매단백매E불민감，대단백매K부분민감，대중국각도구백협고병균적7충치병형도유강렬적억살작용。단백조제액100℃가열15 min후탈염，경DEAE-Sepharose Fast Flow주층석득3개미분개적항균활성봉，미흡부부분경CM-Sepharose Fast Flow주층석，득도1개주활성봉。유우B.subtilis SO113적분비단백대수도백협고병균표현출량호적엄보항성이급봉부적항균활성봉，인차대기진일보적연구화설법극륭편마항균단백적기인도구유중요의의。
In in vitro tests,Bacillus subtilis SO113 showed strongly antibacterial activity against Xanthomonas oryzae pv.oryzae(Xoo)(showing a clear inhibition zone,anti-Xoo).A crude antibacterial preparation(CAP)was obtained by adding ammonium sulphate to supernatant fluid of B.subtilis SO113 to a final saturation of 60% and displayed strong killing activity to 7 pathotypes of Xoo which covers all rice cropping-region in China.The anti-Xoo activity of CAP is thermostable,resistant to p roteinase E,and partially sensitive to proteinase K.One and three peaks with anti-Xoo activity were obtained from CAP, which previously heated at 100℃ for 15 min and salts was removed from, after DEAE-Sepharose Fast Flow and CM-Sepharose Fast Flow, respectively. It is significant to clone the genes encoding those anti-Xoo peptides because the anti-Xoo peptides from B.subtilis SO113 showed extensively inhibitory activities against 7 pathotypes of Xoo in China.