解剖学杂志
解剖學雜誌
해부학잡지
CHINESE JOURNAL OF ANATOMY
2001年
1期
24-27
,共4页
徐丹令%李广君%倪之挺
徐丹令%李廣君%倪之挺
서단령%리엄군%예지정
海马%谷氨酸%兴奋毒性%大鼠
海馬%穀氨痠%興奮毒性%大鼠
해마%곡안산%흥강독성%대서
目的:为明确谷氨酸对神经细胞有兴奋性和毒性作用。方法:选用具有较多N-甲基D-天门冬氨酸(NMDA)受体的海马作为研究对象,选用SD大鼠,给予L谷氨酸钠盐(MSG)皮下注射,在不同的时间内观察大鼠的行为表现和其海马神经细胞受损的形态学的改变。结果:发现大鼠的早期行为表现较为兴奋,而后随时间的延长表现为迟钝。在光镜和电镜下,神经细胞早期为水肿,后期为水肿明显和细胞器的破坏直至细胞的固缩,损伤主要表现在CA1区。结论:提示边缘系统的海马结构易受谷氨酸的影响,同时也表明了谷氨酸对神经细胞的兴奋毒性作用,可为临床药物和食物添加剂的运用提供一定的依据。
目的:為明確穀氨痠對神經細胞有興奮性和毒性作用。方法:選用具有較多N-甲基D-天門鼕氨痠(NMDA)受體的海馬作為研究對象,選用SD大鼠,給予L穀氨痠鈉鹽(MSG)皮下註射,在不同的時間內觀察大鼠的行為錶現和其海馬神經細胞受損的形態學的改變。結果:髮現大鼠的早期行為錶現較為興奮,而後隨時間的延長錶現為遲鈍。在光鏡和電鏡下,神經細胞早期為水腫,後期為水腫明顯和細胞器的破壞直至細胞的固縮,損傷主要錶現在CA1區。結論:提示邊緣繫統的海馬結構易受穀氨痠的影響,同時也錶明瞭穀氨痠對神經細胞的興奮毒性作用,可為臨床藥物和食物添加劑的運用提供一定的依據。
목적:위명학곡안산대신경세포유흥강성화독성작용。방법:선용구유교다N-갑기D-천문동안산(NMDA)수체적해마작위연구대상,선용SD대서,급여L곡안산납염(MSG)피하주사,재불동적시간내관찰대서적행위표현화기해마신경세포수손적형태학적개변。결과:발현대서적조기행위표현교위흥강,이후수시간적연장표현위지둔。재광경화전경하,신경세포조기위수종,후기위수종명현화세포기적파배직지세포적고축,손상주요표현재CA1구。결론:제시변연계통적해마결구역수곡안산적영향,동시야표명료곡안산대신경세포적흥강독성작용,가위림상약물화식물첨가제적운용제공일정적의거。
To understand the excitotoxic effect of glutamate on the neurons. Methods: SD rats were used to study its response and the hippocampus micromorphology after treated with monosdium L-glutamate(MSG) subcutaneously. Results: It showed that the rats were excited at the early stage and were sluggish at late stage. The morphology (under the light and electron microscopies) showed that the neurons of hippocampus being observed had swollen cytoplasm slightly at early stage,edematous changes were obviously and pyknotic nuclei of neurons were observed at the late stage in the CA1 range. Conculsion: The structure of hippocampus is influenced by Glutamate has an exicitotoxic effect on neurons. It may provide scientific basis for pharmacology and food additives.