CAJ | 학술논문

目的：研究细胞凋亡相关基因bcl-x在人鼻咽癌高分化上皮细胞株CNE-1和低分化上皮细胞株CNE-2Z中的转录和表达。方法：分别抽提两种细胞的总RNA，通过逆转录PCR（RT-PCR）检测bcl-x基因的转录；同时制备两种细胞的蛋白质样品，经免疫印迹检测bcl-x基因的表达。结果：在两株鼻咽癌细胞中均检测到bcl-xL的转录和表达，但没有bcl-xs的表达。结论：bcl-xL在人鼻咽癌高、低分化上皮细胞株中均有表达，但表达量没有明显差异提示其表达可能与细胞分化程度无关。
목적：연구세포조망상관기인bcl-x재인비인암고분화상피세포주CNE-1화저분화상피세포주CNE-2Z중적전록화표체。방법：분별추제량충세포적총RNA，통과역전록PCR（RT-PCR）검측bcl-x기인적전록；동시제비량충세포적단백질양품，경면역인적검측bcl-x기인적표체。결과：재량주비인암세포중균검측도bcl-xL적전록화표체，단몰유bcl-xs적표체。결론：bcl-xL재인비인암고、저분화상피세포주중균유표체，단표체량몰유명현차이제시기표체가능여세포분화정도무관。
Objective：To study the transcription and expression of apoptosis-related gene bcl-x in two cell lines of human nasopharyngeal carcinoma（NPC）．Methods：Total RNA was isolated from NPC cells in high differentiation（CNE-1）and low differentiation（CNE-2z）cell lines with Trizol kit．Transcription and protein level of bcl-x were detected by RT-PCR and western blot，respectively．Results：transcription and expression of bcl-xL were detected in both cell lines，while those of bcl-xS were not．Conclusion：Bcl-xLwas expressed in high and low differentiation NPC cell lines，but its expression may be unrelated to the degree of cell differentiation．