CAJ | 학술논문

以相应引物经PCR扩增了太平洋牡蛎(Crassostrea gigas)的核糖体转录间区域(ITS-1和ITS-2)及线粒体16S rDNA和COI基因片段.PCR产物经T-载体连接后进行克隆和测序,分别得到长度为543、791、530和700 bp的核苷酸序列.4 个DNA片段的A、T、G和C碱基含量分别为23.57%、20.07%、29.47%和26.89%(ITS-1),27.43%、19.22%、27.05%和26.30%(ITS-2),29.25%、29.25%、23.02%和18.49%(16S rDNA),22.71%、39.43%、20.43%和 17.43%(COI).实验证明ITS-1和ITS-2引物在贝类中通用性良好.文中同时讨论了4个序列在我国几种牡蛎的种类鉴别及相关研究的应用潜力.
이상응인물경PCR확증료태평양모려(Crassostrea gigas)적핵당체전록간구역(ITS-1화ITS-2)급선립체16S rDNA화COI기인편단.PCR산물경T-재체련접후진행극륭화측서,분별득도장도위543、791、530화700 bp적핵감산서렬.4 개DNA편단적A、T、G화C감기함량분별위23.57%、20.07%、29.47%화26.89%(ITS-1),27.43%、19.22%、27.05%화26.30%(ITS-2),29.25%、29.25%、23.02%화18.49%(16S rDNA),22.71%、39.43%、20.43%화 17.43%(COI).실험증명ITS-1화ITS-2인물재패류중통용성량호.문중동시토론료4개서렬재아국궤충모려적충류감별급상관연구적응용잠력.
The ribosomal internal transcribed spacer regions (ITS-1 and ITS-2) and mitochondrial gene fragments ( 16S rDNA and COI) of Crassostrea gigas were amplified via PCR, and the PCR products were ligated into T-vectors, cloned and sequenced.The nucleotide sequences of 543,791,530 and 700 bp from ITS-1,ITS-2,partial 16S rRNA gene and partial COI gene were obtained, respectively. The contents of A, T, G and C were 23.57%, 20.07%, 29.47% and 26.89% in ITS-1, 27.43%, 19.22%, 27.05% and 26.30% in ITS-2, 29.25%, 29.25% 23.02% and 18.49% in 16S rDNA, and 22.71%, 39.43%, 20.43% and 17.43% in COI in turn. The primers of ITS-1 and ITS-2 proved to be very universal in a variety of mollusk species. The potential uses of these four sequences for species identification and relevant research of several closely related oyster species were discussed.