CAJ | 학술논문

目的:旨在研究新疆柯尔克孜族HLA-DRB1位点等位基因的多态性.方法:采用聚合酶链反应-序列特异引物法(PCR-SSP)对新疆地区柯尔克孜族85名健康无关个体进行HLA-DRB1等位基因分型.结果:共检出HLA-DRB1位点等位基因14种,其中高频率的等位基因是HLA-DRB1*03 (0.3769)、 HLA-DRB1*11 (0.1391)、 HLA-DRB1*07 (0.1056),低频率的等位基因是HLA-DRB1*08 (0.0059)、 HLA-DRB1*01 (0.0178)、 HLA-DRB1*1302 (0.0178)、 HLA-DRB1*1401 (0.0118).柯尔克孜族的HLA-DRB1基因频率分布具有中国北方人群的特点,尤其与西北地区汉族和蒙古族人群最为接近(0.156 、 0.237). 结论:提供了新疆地区柯尔克孜族人HLA-DRB1的14种等位基因的基因频率,为该民族的人类学及疾病相关研究提供了参考数据.
목적:지재연구신강가이극자족HLA-DRB1위점등위기인적다태성.방법:채용취합매련반응-서렬특이인물법(PCR-SSP)대신강지구가이극자족85명건강무관개체진행HLA-DRB1등위기인분형.결과:공검출HLA-DRB1위점등위기인14충,기중고빈솔적등위기인시HLA-DRB1*03 (0.3769)、 HLA-DRB1*11 (0.1391)、 HLA-DRB1*07 (0.1056),저빈솔적등위기인시HLA-DRB1*08 (0.0059)、 HLA-DRB1*01 (0.0178)、 HLA-DRB1*1302 (0.0178)、 HLA-DRB1*1401 (0.0118).가이극자족적HLA-DRB1기인빈솔분포구유중국북방인군적특점,우기여서북지구한족화몽고족인군최위접근(0.156 、 0.237). 결론:제공료신강지구가이극자족인HLA-DRB1적14충등위기인적기인빈솔,위해민족적인류학급질병상관연구제공료삼고수거.
Objective: To investigate polymorphism of HLA-DRB1 in Khalkhas of Xinjiang. Methods: Polymerase chain reaction with sequence specific primer (PCR-SSP) was used to determine HLA-DRB1 genotypes of 85 unrelated healthy Khalkhas of Xinjiang, compared with other different ethnic groups mainly from northwest of China. Results: 14 alleles of HLA-DRB1 were detected. The allele frequency of HLA-DRB1*03 (0.3759) was the highest;The allele frequencies of HLA-DRB1*11 (0.1391), HLA-DRB1*07 (0.1056) and HLA-DRB1*15 (0.1056) were higher;The allele frequency of HLA-DRB1*08 (0.0059), HLA-DRB1*01 (0.0178), HLA-DRB1*1302 (0.0178) and HLA-DRB1*1401 (0.0118) were low, and HLA-DRB1*08 was the lowest. The overall allele distribution profile on HLA-DRB1 of the Khalkhas had the same characteristic as that of northern groups in China, and was much dramatically close to that of Mongolia and northwest Hans. Conclusion: This study has provided 14 normal alleles frequencies of HLA-DRB1 on Xinjiang Khalkhas, which is of important reference value for studies on anthropology and related illnesses in Khalkhas nationality.