CAJ | 학술논문

为研究沙冬青脱水素的功能,根据沙冬青cDNA文库中脱水素基因序列设计特异引物,采用PCR技术扩增脱水素基因的DNA全长,并对其内部的一个碱基进行了定点突变,然后成功地将其连接到pCAMBIA3301载体中,用冻融法将该表达载体质粒导入根癌农杆菌菌株LBA4404中,通过农杆菌介导法,对糖用甜菜进行了遗传转化,获得了Km抗性的甜菜植株,生根后移栽到花盆中.
위연구사동청탈수소적공능,근거사동청cDNA문고중탈수소기인서렬설계특이인물,채용PCR기술확증탈수소기인적DNA전장,병대기내부적일개감기진행료정점돌변,연후성공지장기련접도pCAMBIA3301재체중,용동융법장해표체재체질립도입근암농간균균주LBA4404중,통과농간균개도법,대당용첨채진행료유전전화,획득료Km항성적첨채식주,생근후이재도화분중.
In order to study function of Ammopiptanthus mongolicus dehydrin gene,First,the primers were designed according to the sequence of Ammopiptanthus mongolicus dehydrin cDNA library,the dehydrin gene was amplified with PCR,then carried out site-directed mutagenesis for its a base internal,and cloned into pCAMBIA3301 vector.The dehydrin gene was introduced into sugar beet by Agrobacterium mediated tumerfaciens,and resistance to Km of Sugar beet were obtained,which lays a good foundation for the further research.