CAJ | 학술논문

本文应用电子吸收光谱、溴化乙锭荧光光谱、粘度测定和琼脂糖凝胶电泳方法研究了甘氨酰甘氨酸-铜(Ⅱ)-芳香氮碱型配合物:[Cu(Gly-glY)(TATP)]·2H_2O(1)及[Cu(Gly-gly)(Phen)]·3H_2O(2)(Gly-Gly=甘氨酰甘氨酸,TATP=1,4,8,9-四氮三联苯,Phen=1,10-邻菲咯啉)与DNA之间的相互作用.结果表明,这些配合物通过插入作用与DNA结合,结合能力大小为:配合物1>2;在维生素C存在下对pBR322 DNA具有显著的氧化断裂作用;活性大小为:配合物1>2.
본문응용전자흡수광보、추화을정형광광보、점도측정화경지당응효전영방법연구료감안선감안산-동(Ⅱ)-방향담감형배합물:[Cu(Gly-glY)(TATP)]·2H_2O(1)급[Cu(Gly-gly)(Phen)]·3H_2O(2)(Gly-Gly=감안선감안산,TATP=1,4,8,9-사담삼련분,Phen=1,10-린비각람)여DNA지간적상호작용.결과표명,저사배합물통과삽입작용여DNA결합,결합능력대소위:배합물1>2;재유생소C존재하대pBR322 DNA구유현저적양화단렬작용;활성대소위:배합물1>2.
The interaction of copper(Ⅱ)complexes: [Cu (Gly-gly) (TATP)] · 2H_2O (1)) and [Cu (Gly-gly) (Phen)] · 3H_2O (2) ((Gly-gly = Glycyl-glycine,TATP = 1,4,8,9-tetranitrogen Phen = 1,10-phenanthroline) with DNA has been investigated by electronic absorption spectroscopy, ethidium bromide(EB) fluorescence spectroscopy, viscosity and agarose gel electrophoresis measurements. The results indicated that the complexes could bind to DNA by intercalatiive mode, and cleave pBR322 DNA in the presence of vitamin C with the order of the binding ability and cleavage activity: complex 1 > 2.