CAJ | 학술논문

用一个采自武汉地区的甜菜夜蛾田间种群(N)和一个相对敏感种群(CK),比较了甜菜夜蛾的表皮酚氧化酶(PPO)、乙酰胆碱酯酶(TCE)、谷胱甘肽-S转移酶(GST)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)酶活力差异.用舍有0.14 mg/kg(致死中量)虫酰肼的带毒饲料处理CK种群的甜菜夜蛾,处理后24、48 h和72 h测定酶活力,结果与CK种群相比,PPO酶活力分别降低79.12%、10.54%和56.23%;SOD酶活力分别上升71.59%、63.16%和79.55%;GST酶活力在处理后24 h酶活力上升41.43%.N种群与CK种群比,PPO酶活力降低38.47%;GST酶活力升高34.20%.采用PAGE凝胶电泳方法比较了CK、N和用虫酰肼分别汰选CK种群4代和6代的甜菜夜蛾种群酯酶同工酶谱差异.结果表明,CK共有15条酯酶同工酶带,N和汰选种群均只有10条带.研究结果初步表明甜菜夜蛾对虫酰肼的抗药性可能与PPO、GST、SOD、EI酶活力变化有关,与CAT关系不大,与TCE无关.
용일개채자무한지구적첨채야아전간충군(N)화일개상대민감충군(CK),비교료첨채야아적표피분양화매(PPO)、을선담감지매(TCE)、곡광감태-S전이매(GST)、초양화물기화매(SOD)화과양화경매(CAT)매활력차이.용사유0.14 mg/kg(치사중량)충선정적대독사료처리CK충군적첨채야아,처리후24、48 h화72 h측정매활력,결과여CK충군상비,PPO매활력분별강저79.12%、10.54%화56.23%;SOD매활력분별상승71.59%、63.16%화79.55%;GST매활력재처리후24 h매활력상승41.43%.N충군여CK충군비,PPO매활력강저38.47%;GST매활력승고34.20%.채용PAGE응효전영방법비교료CK、N화용충선정분별태선CK충군4대화6대적첨채야아충군지매동공매보차이.결과표명,CK공유15조지매동공매대,N화태선충군균지유10조대.연구결과초보표명첨채야아대충선정적항약성가능여PPO、GST、SOD、EI매활력변화유관,여CAT관계불대,여TCE무관.
Activities of polyphenoloxidase (PPO), true choline esterase (TCE), glutathione-S-transferase (GST),superoxide dismutase (SOD) and catalase (CAT) of the beet armyworm, Spodoptera exigua (Hubner), were test-ed in vivo in a relatively susceptible population (CK) kept in the laboratory and a population (N) collected from vegetable field in Wuhan. The CK population was treated with 0.14 mg/kg(LC_(50)) tebufenozide. At 24 h, 48 h and 72 h after treatment, the activities of PPO decreased by 79.12%, 10.54% and 56.23%, respectively. And SOD activities increased by 71.59%, 63.16% and 79.55%, respectively. The GST activities increased by 41.43% after 24 h treatment. Compared with the CK population, PPO activity of the N population decreased by 38.47% and GST activity increased by 34.20%. Using PAGE method, the esterase isoenzymes in CK population, N popu-lation and the tebufenozide-selected populations of four and six times, were determined. The results indicated that there were 15 isozyme bands in CK population, but 10 bands in N and the two selected populations. Preliminary results showed that tebufenozide resistance in the beet armyworm might be related to the PPO, GST, SOD and EI, but not to the CAT and TCE.