国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2012年
17期
1305-1309
,共5页
肺气肿%自体免疫%基质金属蛋白酶9%基质金属蛋白酶12%干扰素介导蛋白10
肺氣腫%自體免疫%基質金屬蛋白酶9%基質金屬蛋白酶12%榦擾素介導蛋白10
폐기종%자체면역%기질금속단백매9%기질금속단백매12%간우소개도단백10
Emphysema%Autoimmunity%Matrix Metalloproteinase-9%Matrix Metalloproteinase-12%Interferon-inducible protein-10
目的 通过弹性蛋白主动免疫及熏烟制造豚鼠肺气肿模型,观察各模型中肺组织和心肌组织基质金属蛋白酶9(MMP-9)、MMP - 12及干扰素介导蛋白10(IP-10)的表达情况及组织破坏情况,探究MMP-9 、MMP- 12及IP- 10在自体免疫反应所致肺气肿形成过程中的作用.方法 将24只雄性豚鼠随机分为3组:主动免疫组、熏烟组、对照组.主动免疫组于皮下多点注射弹性蛋白；熏烟组采用传统熏烟造模；对照组不做任何处理.造模满12周后进行肺组织及心肌组织的病理形态观察.并用免疫组织化学法观察各组组织中的MMP-9 、MMP-12及IP-10蛋白的表达情况.结果 主动免疫组及熏烟组肺组织及心肌组织中MMP -9、MMP-12及1P-10均较对照组增多(P＜0.05).主动免疫组肺组织中MMP-9、MMP -12的蛋白表达与熏烟组差异无统计学意义(P＞0.05).主动免疫组心肌组织中MMP-9、MMP- 12及IP -10较熏烟组明显增多(P＜0.05).结论 弹性蛋白抗体自体免疫可以诱发豚鼠肺气肿的形成.MMP-9、MMP -12及IP-10在自体免疫反应所致肺气肿的发生发展中有重要的促进作用.
目的 通過彈性蛋白主動免疫及熏煙製造豚鼠肺氣腫模型,觀察各模型中肺組織和心肌組織基質金屬蛋白酶9(MMP-9)、MMP - 12及榦擾素介導蛋白10(IP-10)的錶達情況及組織破壞情況,探究MMP-9 、MMP- 12及IP- 10在自體免疫反應所緻肺氣腫形成過程中的作用.方法 將24隻雄性豚鼠隨機分為3組:主動免疫組、熏煙組、對照組.主動免疫組于皮下多點註射彈性蛋白；熏煙組採用傳統熏煙造模；對照組不做任何處理.造模滿12週後進行肺組織及心肌組織的病理形態觀察.併用免疫組織化學法觀察各組組織中的MMP-9 、MMP-12及IP-10蛋白的錶達情況.結果 主動免疫組及熏煙組肺組織及心肌組織中MMP -9、MMP-12及1P-10均較對照組增多(P＜0.05).主動免疫組肺組織中MMP-9、MMP -12的蛋白錶達與熏煙組差異無統計學意義(P＞0.05).主動免疫組心肌組織中MMP-9、MMP- 12及IP -10較熏煙組明顯增多(P＜0.05).結論 彈性蛋白抗體自體免疫可以誘髮豚鼠肺氣腫的形成.MMP-9、MMP -12及IP-10在自體免疫反應所緻肺氣腫的髮生髮展中有重要的促進作用.
목적 통과탄성단백주동면역급훈연제조돈서폐기종모형,관찰각모형중폐조직화심기조직기질금속단백매9(MMP-9)、MMP - 12급간우소개도단백10(IP-10)적표체정황급조직파배정황,탐구MMP-9 、MMP- 12급IP- 10재자체면역반응소치폐기종형성과정중적작용.방법 장24지웅성돈서수궤분위3조:주동면역조、훈연조、대조조.주동면역조우피하다점주사탄성단백；훈연조채용전통훈연조모；대조조불주임하처리.조모만12주후진행폐조직급심기조직적병리형태관찰.병용면역조직화학법관찰각조조직중적MMP-9 、MMP-12급IP-10단백적표체정황.결과 주동면역조급훈연조폐조직급심기조직중MMP -9、MMP-12급1P-10균교대조조증다(P＜0.05).주동면역조폐조직중MMP-9、MMP -12적단백표체여훈연조차이무통계학의의(P＞0.05).주동면역조심기조직중MMP-9、MMP- 12급IP -10교훈연조명현증다(P＜0.05).결론 탄성단백항체자체면역가이유발돈서폐기종적형성.MMP-9、MMP -12급IP-10재자체면역반응소치폐기종적발생발전중유중요적촉진작용.
Objective To make emphysema models by active immunization and cigarette smoking using guinea pigs,then observe the expression of MMP-9,MMP-12,IP-10 and the destruction of pulmonary tissue and myocardial tissue in each model,to explore the role of the MMP-9,MMP-12,IP-10in the emphysema which induced by active immunization.Methods The guinea pigs were randomly divided into three groups,involving active immunization group,smoking group and control group.The active immunization group used the method of subcutaneous injection of elastin at multiply points and the smoking group used the traditional method,while the control group performed nothing.To observe the morphosis of pulmonary tissue and myocardial tissue and the expression of MMP-9,MMP -12,IP-10 in the pulmonary tissue and myocardial tissue of every model were observed by immunohistochemical staining,Results The expression of MMP-9,MMP-12,IP -10 in the pulmonary tissue and myocardial tissue has both significantly increased in the active immunization group and the smoking group,compared with the control group ( P ＜0.05).The expression of MMP-9,MMP-12 in the pulmonary tissue in the active immunization group compared with the smoking group,and there is no obvious difference ( P ＞0.05).The expression of matrix MMP 9,MMP-12 in the myocardial tissue in the active immunization group,compared with the smoking group ( P ＜ 0.05).Conclusions Emphysema model of guinea pig can be established by active immunization.MMP-9,MMP-12,IP- 10 has important positive influence on emphysema which induced by active immunization.
