中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2011年
1期
13-16
,共4页
冯广忠%邢琳%侯勇生%傅少颖
馮廣忠%邢琳%侯勇生%傅少穎
풍엄충%형림%후용생%부소영
CD4+CD25+T细胞%实验性自身免疫性葡萄膜视网膜炎%自动免疫
CD4+CD25+T細胞%實驗性自身免疫性葡萄膜視網膜炎%自動免疫
CD4+CD25+T세포%실험성자신면역성포도막시망막염%자동면역
CD4+CD25+T cell%Experimental autoimmune uveoretinitis%Autoimmune
背景 实验性自身免疫性葡萄膜视网膜炎(EAU)已被证明是一种由T淋巴细胞介导的器官特异性自限性疾病.研究表明CD4+CD25+T细胞可能参与了EAU的调控,但其作用机制尚有待研究.目的 探讨EAU中CD4+CD25+T细胞的表达变化.方法 按照Caspi的方法提纯牛视网膜S抗原,与弗氏完全佐剂混合后,于24只Lewis大鼠右后足底部注射0.1 ml制作EAU模型,4只未处理大鼠作为正常对照组.免疫后每日用裂隙灯显微镜观察大鼠眼部变化.造模后7、12、15、21 d处死动物,取大鼠视网膜、引流淋巴结、脾脏,对大鼠眼组织切片进行苏木精-伊红染色并进行病理评分.对各时间点收集的大鼠视网膜、引流淋巴结、脾脏分别制备单细胞悬液,流式细胞仪检测各时间点3种组织中CD4+CD25+T细胞的表达情况.结果 造模7 d后大鼠睫状充血,虹膜血管扩张;15 d后炎症达高峰,前房大量炎性渗出;21 d后炎症消退.眼部病理评分结果与临床所见一致,造模15 d组病理评分与其他各组比较差异均有统计学意义(P=0.000).正常对照组大鼠脾脏和淋巴结中有2.0%CD4+T细胞表达CD25,造模组CD4+CD25+T细胞表达增加,并在EAU高峰期达最高,EAU消退期轻微下降,与正常对照组比较差异有统计学意义(P=0.000).结论 CD4+CD25+T细胞在EAU动物模型炎症组织中表达的动态变化与炎症反应有关,提示CD4+CD25+T细胞参与EAU的发生发展和消退过程.
揹景 實驗性自身免疫性葡萄膜視網膜炎(EAU)已被證明是一種由T淋巴細胞介導的器官特異性自限性疾病.研究錶明CD4+CD25+T細胞可能參與瞭EAU的調控,但其作用機製尚有待研究.目的 探討EAU中CD4+CD25+T細胞的錶達變化.方法 按照Caspi的方法提純牛視網膜S抗原,與弗氏完全佐劑混閤後,于24隻Lewis大鼠右後足底部註射0.1 ml製作EAU模型,4隻未處理大鼠作為正常對照組.免疫後每日用裂隙燈顯微鏡觀察大鼠眼部變化.造模後7、12、15、21 d處死動物,取大鼠視網膜、引流淋巴結、脾髒,對大鼠眼組織切片進行囌木精-伊紅染色併進行病理評分.對各時間點收集的大鼠視網膜、引流淋巴結、脾髒分彆製備單細胞懸液,流式細胞儀檢測各時間點3種組織中CD4+CD25+T細胞的錶達情況.結果 造模7 d後大鼠睫狀充血,虹膜血管擴張;15 d後炎癥達高峰,前房大量炎性滲齣;21 d後炎癥消退.眼部病理評分結果與臨床所見一緻,造模15 d組病理評分與其他各組比較差異均有統計學意義(P=0.000).正常對照組大鼠脾髒和淋巴結中有2.0%CD4+T細胞錶達CD25,造模組CD4+CD25+T細胞錶達增加,併在EAU高峰期達最高,EAU消退期輕微下降,與正常對照組比較差異有統計學意義(P=0.000).結論 CD4+CD25+T細胞在EAU動物模型炎癥組織中錶達的動態變化與炎癥反應有關,提示CD4+CD25+T細胞參與EAU的髮生髮展和消退過程.
배경 실험성자신면역성포도막시망막염(EAU)이피증명시일충유T림파세포개도적기관특이성자한성질병.연구표명CD4+CD25+T세포가능삼여료EAU적조공,단기작용궤제상유대연구.목적 탐토EAU중CD4+CD25+T세포적표체변화.방법 안조Caspi적방법제순우시망막S항원,여불씨완전좌제혼합후,우24지Lewis대서우후족저부주사0.1 ml제작EAU모형,4지미처리대서작위정상대조조.면역후매일용렬극등현미경관찰대서안부변화.조모후7、12、15、21 d처사동물,취대서시망막、인류림파결、비장,대대서안조직절편진행소목정-이홍염색병진행병리평분.대각시간점수집적대서시망막、인류림파결、비장분별제비단세포현액,류식세포의검측각시간점3충조직중CD4+CD25+T세포적표체정황.결과 조모7 d후대서첩상충혈,홍막혈관확장;15 d후염증체고봉,전방대량염성삼출;21 d후염증소퇴.안부병리평분결과여림상소견일치,조모15 d조병리평분여기타각조비교차이균유통계학의의(P=0.000).정상대조조대서비장화림파결중유2.0%CD4+T세포표체CD25,조모조CD4+CD25+T세포표체증가,병재EAU고봉기체최고,EAU소퇴기경미하강,여정상대조조비교차이유통계학의의(P=0.000).결론 CD4+CD25+T세포재EAU동물모형염증조직중표체적동태변화여염증반응유관,제시CD4+CD25+T세포삼여EAU적발생발전화소퇴과정.
Background Experimental autoimmune uveoretinitis(EAU)is proved to be an organ-specific,T lymphocyte-mediated autoimmune and self-limited disease.Research showed that CD4+CD25+T cell may play important regulation on the course of events,but its mechanism is pending for further study. Objective Present study was to investigate the potential role of CD4+CD25+T cell in the pathogenesis of EAU. Methods Retinal Santigen(S-Ag)was isolated from bovine retinas according tO the procedure as Caspi's previously describe.0.1 ml Santigen(50μg)emulsified with complete Freund'S adjuvant was injected on footpad of 24 inbred adult female Lewis rats aged six to eight-week-old to induce EAU,and 4 normal Lewis rats were as normal control group.Slim-lamp examination was performed to observe the ocular manifestation.Retinal section was prepared in 7,12,15,21 days aher injection for the pathological examination.The pathological grading was on the lnoki'S method.The retinas,inguinal nodes and spleens of rats were obtained in 7,12,15,21 days after injection and the cellular suspension was prepared.Expression of CD4+CD25+T cells on cellular suspension was assayed using flow eytometry.This study complied with the Standard of Association for Research in Vision and Ophthalmology. ResuRs The obvious inflammatory response of the anterior segment was found in S-Ag injected eyes from 7 days through 21 days.The most serious inflammation was found in 12-15 days under the slim-lamp.The hemotoxylin and eosin staining showed the higher pathological grading from 12 to 15 days after injection,showing significant difference in comparison with 7 days and 14 days(P=0.000).In EAU model rats,expressions of CD4+CD25+T cells was significantly increased in retinas, inguinal nodes and spleens in 15 days after injection,showing evidently differences in comparison with control rats(P=0.000). Conclusion The expression level of CD4+CD25+T cells in inflammatory tissue is associated with the inflammation procedure in EAU model rats.This study indicates that CD4+CD25+T cells may play a role in the development of EAU.
