中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2010年
4期
418-422
,共5页
糖尿病视网膜病变%槲皮素%单核细胞趋化蛋白-1
糖尿病視網膜病變%槲皮素%單覈細胞趨化蛋白-1
당뇨병시망막병변%곡피소%단핵세포추화단백-1
Diabetic retinopathy%Monocyte chemoattractant proein-1%Quercetin
目的 探讨槲皮素干预糖尿病大鼠视网膜病变的机制.方法 清洁雄性Wistar大鼠,随机分为正常对照组和糖尿病动物组,将成模大鼠随机分为糖尿病组,导升明组,槲皮素组.治疗20周.观察糖尿病大鼠一般情况变化,视网膜血管铺片观察毛细血管内皮细胞数(E)和周细胞数(P),并计算E/P比值,免疫组织化学法观察MCP-1在视网膜的表达,ELISA法检测玻璃体内MCP-1浓度.结果 20周时,与糖尿病阴性对照组相比,槲皮素治疗组体重明显增加,周细胞数明显增多,E/P值明显降低.与导升明阳性对照组相比,槲皮素治疗组体重、周细胞数和E/P值无明显差异.20周时,正常对照组视网膜血管未见MCP-1阳性细胞表达糖尿病组走形迂曲的视网膜毛细血管壁上有大量MCP-1阳性细胞表达,而槲皮素和导升明组视煳膜血管铺片MCP-1表达情况与糖尿病组相比,无明显差别.20周时,正常大鼠玻璃体内存在少量MCP-1,糖尿病组、导升明组和槲皮素组大鼠玻璃体内含有大量MCP-1,相互之间无明显差异.结论 槲皮素对实验性大鼠视网膜病变有一定的治疗作用,但其作用不是通过抑制炎症介质MCP-1表达实现的.
目的 探討槲皮素榦預糖尿病大鼠視網膜病變的機製.方法 清潔雄性Wistar大鼠,隨機分為正常對照組和糖尿病動物組,將成模大鼠隨機分為糖尿病組,導升明組,槲皮素組.治療20週.觀察糖尿病大鼠一般情況變化,視網膜血管鋪片觀察毛細血管內皮細胞數(E)和週細胞數(P),併計算E/P比值,免疫組織化學法觀察MCP-1在視網膜的錶達,ELISA法檢測玻璃體內MCP-1濃度.結果 20週時,與糖尿病陰性對照組相比,槲皮素治療組體重明顯增加,週細胞數明顯增多,E/P值明顯降低.與導升明暘性對照組相比,槲皮素治療組體重、週細胞數和E/P值無明顯差異.20週時,正常對照組視網膜血管未見MCP-1暘性細胞錶達糖尿病組走形迂麯的視網膜毛細血管壁上有大量MCP-1暘性細胞錶達,而槲皮素和導升明組視煳膜血管鋪片MCP-1錶達情況與糖尿病組相比,無明顯差彆.20週時,正常大鼠玻璃體內存在少量MCP-1,糖尿病組、導升明組和槲皮素組大鼠玻璃體內含有大量MCP-1,相互之間無明顯差異.結論 槲皮素對實驗性大鼠視網膜病變有一定的治療作用,但其作用不是通過抑製炎癥介質MCP-1錶達實現的.
목적 탐토곡피소간예당뇨병대서시망막병변적궤제.방법 청길웅성Wistar대서,수궤분위정상대조조화당뇨병동물조,장성모대서수궤분위당뇨병조,도승명조,곡피소조.치료20주.관찰당뇨병대서일반정황변화,시망막혈관포편관찰모세혈관내피세포수(E)화주세포수(P),병계산E/P비치,면역조직화학법관찰MCP-1재시망막적표체,ELISA법검측파리체내MCP-1농도.결과 20주시,여당뇨병음성대조조상비,곡피소치료조체중명현증가,주세포수명현증다,E/P치명현강저.여도승명양성대조조상비,곡피소치료조체중、주세포수화E/P치무명현차이.20주시,정상대조조시망막혈관미견MCP-1양성세포표체당뇨병조주형우곡적시망막모세혈관벽상유대량MCP-1양성세포표체,이곡피소화도승명조시호막혈관포편MCP-1표체정황여당뇨병조상비,무명현차별.20주시,정상대서파리체내존재소량MCP-1,당뇨병조、도승명조화곡피소조대서파리체내함유대량MCP-1,상호지간무명현차이.결론 곡피소대실험성대서시망막병변유일정적치료작용,단기작용불시통과억제염증개질MCP-1표체실현적.
Objective To investigate the efficacy of quercetin on the diabetic rat retinal disease.Methods Sixty-six healthy adult male Wistar rats were randomly divided into normal control (NC) group and diabetes mellitus (DM) group.DM group was further randomized into model group,calcium dobesilate,quercetin group.The treated animals were fed with quercetin,calcium dobesilate.The following examinations were carried out 20 weeks after the treatment: Blood glucose and normal condition; retinal digest preparations,E/P ratio; Immunocytochemistry staining and ELISA.Results After 20 weeks,compared with model group,the weight of calcium dobesilate and quercetin group increased significantly (P＜0.01),the morphological changes of the quercetin and calcium dobesilate groups were less lesions,and the quantity of the pericytes was increased significantly,the expression of MCP-1 in treatment group was similar to model group,the concentration of MCP-1 in treatment groups had no significant difference.Conclusions Quercetin had certain therapeutic effect on the experimental diabetic rat retinal disorders in this study.But quercetin dose not show any suppressive effect on the expression of the inflammatory mediator MCP-1.It strongly suggests other ways for the significant efficacy ofquercetin on the experimental DR in rats.
