中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2009年
10期
601-603
,共3页
陈杰%陈勇%邓腾刚%龚建平
陳傑%陳勇%鄧騰剛%龔建平
진걸%진용%산등강%공건평
肝移植%干扰素Ⅱ型%白细胞介素10%移植物排斥
肝移植%榦擾素Ⅱ型%白細胞介素10%移植物排斥
간이식%간우소Ⅱ형%백세포개소10%이식물배척
Liver transplantation%Interferon type Ⅱ%Interleukin-10%Graft rejection
目的 探讨大鼠肝移植排斥反应时γ干扰素(IFN-γ)及白细胞介素10(IL-10)的表达及意义.方法 采用改良的Kamada"二袖套法"制备大鼠原位肝移植模型,同系移植组供、受者均为SD大鼠;同种异体移植组的供者为Wistar大鼠,受者为SD大鼠;另设假手术组.术后7 d处死动物,观察移植肝脏的组织学变化,检测血清IFN-γ和IL-10的含量,以及移植肝脏内IFN-γ和IL-10 mRNA的表达.结果 同种异体移植组移植肝脏有较多坏死肝细胞,汇管区及中央静脉周围可见以淋巴细胞为主的炎症细胞浸润,胆管上皮细胞可见胞浆空泡变性、核固缩或碎裂,整个肝小叶结构紊乱.同系移植组肝脏组织结构仅有轻度缺血再灌注损伤表现,汇管区有较少炎症细胞浸润,胆管上皮细胞结构和肝小叶结构基本正常.同种异体移植组血清IFN-γ为(386.7±14.4)Pg/ml,明显高于同系移植组的(159.8±16.5)pg/ml(P<0.05);同种异体移植组血清IL-10为(126.3±13.1)pg/ml,明显低于同系移植组的(288.3±17.1)pg/ml(P<0.05).同种异体移植组移植肝组织内IF-γ mRNA表达水平明显高于同系移植组(P<0.05),而IL-10 rnRNA表达水平明显低于同系移植组(P<0.05).结论 大鼠肝移植排斥反应时IFN-γ表达明显升高,IL-10表达明显降低;T_H1/T_H2型细胞因子的动态平衡可能在大鼠肝移植排斥反应中起着重要作用.
目的 探討大鼠肝移植排斥反應時γ榦擾素(IFN-γ)及白細胞介素10(IL-10)的錶達及意義.方法 採用改良的Kamada"二袖套法"製備大鼠原位肝移植模型,同繫移植組供、受者均為SD大鼠;同種異體移植組的供者為Wistar大鼠,受者為SD大鼠;另設假手術組.術後7 d處死動物,觀察移植肝髒的組織學變化,檢測血清IFN-γ和IL-10的含量,以及移植肝髒內IFN-γ和IL-10 mRNA的錶達.結果 同種異體移植組移植肝髒有較多壞死肝細胞,彙管區及中央靜脈週圍可見以淋巴細胞為主的炎癥細胞浸潤,膽管上皮細胞可見胞漿空泡變性、覈固縮或碎裂,整箇肝小葉結構紊亂.同繫移植組肝髒組織結構僅有輕度缺血再灌註損傷錶現,彙管區有較少炎癥細胞浸潤,膽管上皮細胞結構和肝小葉結構基本正常.同種異體移植組血清IFN-γ為(386.7±14.4)Pg/ml,明顯高于同繫移植組的(159.8±16.5)pg/ml(P<0.05);同種異體移植組血清IL-10為(126.3±13.1)pg/ml,明顯低于同繫移植組的(288.3±17.1)pg/ml(P<0.05).同種異體移植組移植肝組織內IF-γ mRNA錶達水平明顯高于同繫移植組(P<0.05),而IL-10 rnRNA錶達水平明顯低于同繫移植組(P<0.05).結論 大鼠肝移植排斥反應時IFN-γ錶達明顯升高,IL-10錶達明顯降低;T_H1/T_H2型細胞因子的動態平衡可能在大鼠肝移植排斥反應中起著重要作用.
목적 탐토대서간이식배척반응시γ간우소(IFN-γ)급백세포개소10(IL-10)적표체급의의.방법 채용개량적Kamada"이수투법"제비대서원위간이식모형,동계이식조공、수자균위SD대서;동충이체이식조적공자위Wistar대서,수자위SD대서;령설가수술조.술후7 d처사동물,관찰이식간장적조직학변화,검측혈청IFN-γ화IL-10적함량,이급이식간장내IFN-γ화IL-10 mRNA적표체.결과 동충이체이식조이식간장유교다배사간세포,회관구급중앙정맥주위가견이림파세포위주적염증세포침윤,담관상피세포가견포장공포변성、핵고축혹쇄렬,정개간소협결구문란.동계이식조간장조직결구부유경도결혈재관주손상표현,회관구유교소염증세포침윤,담관상피세포결구화간소협결구기본정상.동충이체이식조혈청IFN-γ위(386.7±14.4)Pg/ml,명현고우동계이식조적(159.8±16.5)pg/ml(P<0.05);동충이체이식조혈청IL-10위(126.3±13.1)pg/ml,명현저우동계이식조적(288.3±17.1)pg/ml(P<0.05).동충이체이식조이식간조직내IF-γ mRNA표체수평명현고우동계이식조(P<0.05),이IL-10 rnRNA표체수평명현저우동계이식조(P<0.05).결론 대서간이식배척반응시IFN-γ표체명현승고,IL-10표체명현강저;T_H1/T_H2형세포인자적동태평형가능재대서간이식배척반응중기착중요작용.
Objective To explore the expression of IFN-γ and IL-10 in lymphocytes in the process of the graft rejection process following liver transplantation in rats and their biological significances.Methods Orthotopic liver transplantation (OLT) models were established by modified Kamada two-cuff technique.Recipients were divided into three groups.The allograft group underwent OLT from male Wistar-to-Sprague-Dawley (SD) rats.The isograft group of SD-to-SD liver transplantation was complemented with a control group of normal male SD rats that had sham operations.The histological changes were examined,as well as serum and Mrna expression levels of IFN-γand IL-10.Results Serious morphological changes were observed in the allografted hepatic tissue.Many necrotic hepatocytes were seen and vacuolar degeneration of kytoplasm,karyopycnosis even nuclear fragmentation was observed in biliary duct endothelial cells.Many inflammatory cells infiltrated the portal areas,and the architecture of the liver lobules was destroyed.In contrast there were mild morphological changes in isograft hepatic tissue .A few inflammatory cells infiltrated the portal area;the hepatic lobule structure remained and biliary duct endothelial cells were nearly normal.Serum IFN-γ levels (pg/ml)determined by ELISA in the allograft group and isograft group were 386.7±14.4 and 159.8±16.5 respectively(P<0.05);for IL-10,they were 126.3±13.1 and 288.3±17.1,respectively(P<0.05).The semiquantitative reverse-transcriptase polymerase chain reaction assay showed that expression levels of IFN-γand IL-10 Mrna were similar to those in serum.Conclusion The expression of IFN-γ was obviously advanced in graft rejection following liver transplantation in rats and the expression of IL-10 was depressed. The dynamic equilibrium of T_H1/T_H2 cytokines is very important in the process of graft rejection following liver transplantation in rats.
