CAJ | 학술논문

目的壳聚糖与质粒DNA可以层层组装形成多层膜,可用于金属表面载基因涂层.本研究采用表面等离子共振(SPR)技术,实时检测金属表面与壳聚糖、壳聚糖与质粒DNA(pDNA)的相互作用,并分析壳聚糖携载质粒DNA的能力以及壳聚糖-质粒DNA多层膜在液流作用下的稳定性.方法在11-巯基十一羧酸处理过的裸金芯片上自组装不同浓度、不同相对分子量的壳聚糖(50～400 ku)和质粒DNA分子.结果层层组装方法中壳聚糖对质粒的携载量与相对分子量和浓度密切相关,即高浓度、高分子量的壳聚糖可以形成更加厚实的多层膜,并可以携载更多的质粒DNA,自组装形成的多层膜还具有耐液流冲刷性,物理性质稳定.结论采用壳聚糖与治疗基因的多层自组装可以在血管支架上携载质粒DNA,为探索载基因血管支架涂层的构建提供了新的方法和手段.
목적 각취당여질립DNA가이층층조장형성다층막,가용우금속표면재기인도층.본연구채용표면등리자공진(SPR)기술,실시검측금속표면여각취당、각취당여질립DNA(pDNA)적상호작용,병분석각취당휴재질립DNA적능력이급각취당-질립DNA다층막재액류작용하적은정성.방법 재11-구기십일최산처리과적라금심편상자조장불동농도、불동상대분자량적각취당(50～400 ku)화질립DNA분자.결과 층층조장방법중각취당대질립적휴재량여상대분자량화농도밀절상관,즉고농도、고분자량적각취당가이형성경가후실적다층막,병가이휴재경다적질립DNA,자조장형성적다층막환구유내액류충쇄성,물이성질은정.결론 채용각취당여치료기인적다층자조장가이재혈관지가상휴재질립DNA,위탐색재기인혈관지가도층적구건제공료신적방법화수단.
Objective To investigate the technology for layer-by-layer assembly of chitosan and plasmid DNA on metal surface, which has a potential in vascular stent mediated gene delivery. Methods Chitosan and plasmid DNA were loaded on the surface of 11-MUA treated gold chip through layer by layer deposition. The real- time monitoring of interactions of various molecular weight (Mw) chitosan samples with 11-MUA and chitosan with plasmid DNA were evaluated by surface plasmon resonance (SPR). The pDNA loading capacity of chitosan and the stability of the muhilayer under flow condition were profiled. Results The amount of DNA loaded by chitosan on the metal surface was dependent on the concentration and molecular weight of chitosan. Thicker multilayer and higher loading of pDNA were obtained with higher concentration and/or higher molecular weight of chitosan. The multilayer is stable under running buffer flushed through the system. Conclusion The assembled multilayer of chitosan and pDNA is stable on the metal surface and can be used as a new tool for stent mediated gene delivery.