中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2011年
17期
2313-2315
,共3页
贫血,再生障碍性%TNF相关凋亡诱导配体%半胱天冬酶-3
貧血,再生障礙性%TNF相關凋亡誘導配體%半胱天鼕酶-3
빈혈,재생장애성%TNF상관조망유도배체%반광천동매-3
Anemia aplastic%TNF related apoptosis-inducing ligand%Caspase-3
目的 探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)和半胱天冬酶-3（Caspase-3）在再生障碍性贫血(AA)骨髓单个核细胞中表达及其在AA造血干细胞凋亡中的作用。方法 选取15例初诊和治疗后进入缓解期的AA患者、15例骨髓象正常的患者为研究对象，采用流式细胞术检测患者骨髓单个核细胞中TRAIL表达，采用酶标仪检测骨髓单个核细胞Caspase-3的表达。结果 AA初诊时、缓解期和对照组TRAIL蛋白表达分别为(5.94±2.57)％、(2.87±1.72)％和(3.01±2.06)％，Caspase-3蛋白表达分别为(1.3840±0.0236)、(0.8018±0.0126)和(0.7441±0.0112)，AA初诊时TRAIL和Caspase-3蛋白表达显著高于缓解期和对照组，差异均有统计学意义（均P＜0.05）；对照组TRAIL和Caspase-3蛋白表达高于AA缓解期，但差异均无统计学意义（均P＞0.05）。结论 AA患者骨髓单个核细胞中TRAIL和Caspase-3呈高表达，它们可能参与诱导AA患者造血细胞凋亡。
目的 探討腫瘤壞死因子相關凋亡誘導配體(TRAIL)和半胱天鼕酶-3（Caspase-3）在再生障礙性貧血(AA)骨髓單箇覈細胞中錶達及其在AA造血榦細胞凋亡中的作用。方法 選取15例初診和治療後進入緩解期的AA患者、15例骨髓象正常的患者為研究對象，採用流式細胞術檢測患者骨髓單箇覈細胞中TRAIL錶達，採用酶標儀檢測骨髓單箇覈細胞Caspase-3的錶達。結果 AA初診時、緩解期和對照組TRAIL蛋白錶達分彆為(5.94±2.57)％、(2.87±1.72)％和(3.01±2.06)％，Caspase-3蛋白錶達分彆為(1.3840±0.0236)、(0.8018±0.0126)和(0.7441±0.0112)，AA初診時TRAIL和Caspase-3蛋白錶達顯著高于緩解期和對照組，差異均有統計學意義（均P＜0.05）；對照組TRAIL和Caspase-3蛋白錶達高于AA緩解期，但差異均無統計學意義（均P＞0.05）。結論 AA患者骨髓單箇覈細胞中TRAIL和Caspase-3呈高錶達，它們可能參與誘導AA患者造血細胞凋亡。
목적 탐토종류배사인자상관조망유도배체(TRAIL)화반광천동매-3（Caspase-3）재재생장애성빈혈(AA)골수단개핵세포중표체급기재AA조혈간세포조망중적작용。방법 선취15례초진화치료후진입완해기적AA환자、15례골수상정상적환자위연구대상，채용류식세포술검측환자골수단개핵세포중TRAIL표체，채용매표의검측골수단개핵세포Caspase-3적표체。결과 AA초진시、완해기화대조조TRAIL단백표체분별위(5.94±2.57)％、(2.87±1.72)％화(3.01±2.06)％，Caspase-3단백표체분별위(1.3840±0.0236)、(0.8018±0.0126)화(0.7441±0.0112)，AA초진시TRAIL화Caspase-3단백표체현저고우완해기화대조조，차이균유통계학의의（균P＜0.05）；대조조TRAIL화Caspase-3단백표체고우AA완해기，단차이균무통계학의의（균P＞0.05）。결론 AA환자골수단개핵세포중TRAIL화Caspase-3정고표체，타문가능삼여유도AA환자조혈세포조망。
Objective To study the expression and clinical significance of TNF related apoptosis-inducing ligand(TRAIL) and Caspase-3 in the bone marrow mononuclear cell(BMMNC) of aplastic anemia(AA) ,and to approach their effect on the apoptosis of haemopoietic stem cell in AA patients.Methods The TRAIL and Caspase-3 expression in 15 cases with newly diagnosed AA patients and remission patients,and 15 cases with normal bone marrow patients were detected by flow cytometry and microplate reader. Results The TRAIL expression of newly diagnosed AA patient, emission AA patients and control group was ( 5.94 ± 2. 57 ) ％, ( 2. 87 ± 1. 72 ) ％ and ( 3.01 ±2. 06)％ respectively,the Caspase-3 expression of newly diagnosed AA patient, emission AA patients and control group was ( 1. 3840 ± 0. 0236), (0. 8018 ± 0. 0126) and ( 0. 7441 ± 0. 0112 ) respectively. The TRAIL and Caspase-3 expression of newly diagnosed AA patient were significantly higher than the remission AA patients and control group,the difference was significant( all P ＜0. 05) ;The TRAIL and Caspase-3 expression of control group were higher than the remission AA patients, but the difference was not significant ( all P ＞ 0. 05 ). Conclusion The TRAIL and Caspase-3 had high expression in the BMMNC of AA patients, and the apoptosis induced by TRAIL and Caspase-3 should play an important role in the pathogenesis of AA.
