CAJ | 학술논문

从含铬废弃物提取的水解蛋白质与不同量的戊二醛,在不同的温度下反应.用聚酰胺凝胶电泳法(SDS-PAGE)分析了改性蛋白质的分子量及分子量分布.用Canon Manning粘度法测定了改性产物的粘度.实验表明改性产物的分子量随戊二醛的用量增加而增加.最佳的戊二醛用量约为水解胶原质量的12%,反应温度大于60*!℃.同时,用改性产物做了皮革的填充实验,用萤光标记改性胶原,通过扫描电镜研究了填充皮革的纤维结构.研究结果表明,改性产物具有较好的填充性能,采用适当的应用工艺,改性产物可能用作皮革填充剂.
종함락폐기물제취적수해단백질여불동량적무이철,재불동적온도하반응.용취선알응효전영법(SDS-PAGE)분석료개성단백질적분자량급분자량분포.용Canon Manning점도법측정료개성산물적점도.실험표명개성산물적분자량수무이철적용량증가이증가.최가적무이철용량약위수해효원질량적12%,반응온도대우60*!℃.동시,용개성산물주료피혁적전충실험,용형광표기개성효원,통과소묘전경연구료전충피혁적섬유결구.연구결과표명,개성산물구유교호적전충성능,채용괄당적응용공예,개성산물가능용작피혁전충제.
In order to enhance the commercial value of hydrolysate protein from chrome shavings, the chemical modification of hydrolysate protein with glutaraldehyde was studied. Samples of hydrolysate protein extracted from chromium-containing leather waste were chemically modified with different amounts of glutaraldehyde and at different temperatures. Polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze the modified fragments. A Cannon Manning viscosimeter was used to measure the viscosities of modified products. The experiments showed the molecular weights of the modified products increase with the amount of glutaraldehyde. And the better amount of glutaraldehyde based on hydrolysate protein is about 12% and the optimal offer temperature is over 60*!℃.Also, the filling experiments was done with modified products. A fluorescence method was used to label modified product for microscope observation. The scanning electron microscope analyzed the fibrous structure of filled leather. The results show the modified product has better filling properties. With the helps of a proper process, the modified products can be used as filler in tanning industry.