CAJ | 학술논문

为比较不同赤眼蜂酚氧化酶(PO)活性的大小,明确赤眼蜂种间免疫防御能力强弱,本研究通过研磨、离心提取松毛虫赤眼蜂 Trichogramma dendrolimi、螟黄赤眼蜂 T. chilonis、玉米螟赤眼蜂 T. ostriniae 匀浆液,首次测定了几种化学因子(Ca~(2+)、昆布多糖、纤维素、右旋糖酐、脂多糖和丝氨酸蛋白酶抑制剂)对3种供试赤眼蜂匀浆液中酚氧化酶原激活系统(proPO-AS)的激活程度.结果表明:玉米螟赤眼蜂匀浆液PO活性最高,螟黄赤眼蜂次之,松毛虫赤眼蜂最低.Ca~(2+)是激活供试赤眼蜂proPO的必需因子,且在0.03 mol/L浓度下激活作用最强;昆布多糖和脂多糖也能有效激活prorPO, 0.01及0.05 mg/mL昆布多糖能强烈激活PO活性;纤维素和右旋糖酐对PO活性存在显著抑制作用.结果证明了赤眼蜂种间存在明显的免疫能力差异.本研究建立了小型昆虫proPO-AS研究体系,为研究Wolbachia-小型昆虫的免疫互作体系奠定了基础.
위비교불동적안봉분양화매(PO)활성적대소,명학적안봉충간면역방어능력강약,본연구통과연마、리심제취송모충적안봉 Trichogramma dendrolimi、명황적안봉 T. chilonis、옥미명적안봉 T. ostriniae 균장액,수차측정료궤충화학인자(Ca~(2+)、곤포다당、섬유소、우선당항、지다당화사안산단백매억제제)대3충공시적안봉균장액중분양화매원격활계통(proPO-AS)적격활정도.결과표명:옥미명적안봉균장액PO활성최고,명황적안봉차지,송모충적안봉최저.Ca~(2+)시격활공시적안봉proPO적필수인자,차재0.03 mol/L농도하격활작용최강;곤포다당화지다당야능유효격활prorPO, 0.01급0.05 mg/mL곤포다당능강렬격활PO활성;섬유소화우선당항대PO활성존재현저억제작용.결과증명료적안봉충간존재명현적면역능력차이.본연구건립료소형곤충proPO-AS연구체계,위연구Wolbachia-소형곤충적면역호작체계전정료기출.
Prophenoloxidase-activating system (proPO-AS) is one of the most important humoral immunity systems in insects. The crude extracts of Trichogramma ostriniae, T. chilonis and T. dendrolimi were collected by grinding and centrifugation, and PO activities of three Trichogramm species induced by calcium ions, laminarin, dextran, and PMSF were compared. The results indicated that the highest PO activity was found in the crude extract from T. ostriniae, followed by T. chilonis, and then T. dendrolimi. Ca~(2+) was required for PO activation, and the maximal activation was observed at the Ca~(2+) concentration of 0.03 mol/L. Laminarin and lipopoyusaccharide could also effectively activate proPO, and 0.01 and 0.05 mg/mL laminarin caused strong activation. Cellulose and dextran were proved to be significant inhibitors to PO. A model for proPO-AS studies of small insects was established, which provides an experimental basis for studying the interaction of immune system of symbiotic bacteria-small insects.