中国医科大学学报
中國醫科大學學報
중국의과대학학보
JOURNAL OF CHINA MEDICAL UNIVERSITY
2010年
1期
1-3
,共3页
于雪馨%王常林%孙荣国%杨屹
于雪馨%王常林%孫榮國%楊屹
우설형%왕상림%손영국%양흘
输尿管梗阻%足细胞表型%肾脏损伤
輸尿管梗阻%足細胞錶型%腎髒損傷
수뇨관경조%족세포표형%신장손상
unilateral ureteral obstruction%pedocyte cell transition%renal injury
目的 观察鼠输尿管完全梗阻后肾脏病理和足细胞袁型改变的时序变化,寻找输尿管梗阻后肾脏发生不可逆损伤的时相.方法 建立单侧输尿管完全梗阻动物模型,梗阻后不同时间点取出肾脏,经HE染色和免疫荧光双标方法,观察肾脏病理及足细胞表型的改变.结果 大体观察,梗阻24 h后出现肾孟扩张,梗阻3 d后出现肾皮质变薄.组织学观察,梗阻12 h后出现一部分近曲小管扩张,小管上皮细胞呈水样变性肿胀,在梗阻3 d后出现大量小管上皮细胞变形肿胀.免疫荧光双标法,梗阻24 h后有少量足细胞、巨噬细胞同时被2种抗体标记,2 d后足细胞呈减少趋势,而巨噬细胞逐渐增多.结论 输尿管梗阻后在肾脏病理改变早于肾脏发生形态学改变的发生,输尿管梗阻后2A h发生足细胞表型的改变,与肾脏肾盂出现明显扩张的时间相近,随着梗阻时间的延长,肾脏病理改变逐渐加重,足细胞转化逐渐增多.
目的 觀察鼠輸尿管完全梗阻後腎髒病理和足細胞袁型改變的時序變化,尋找輸尿管梗阻後腎髒髮生不可逆損傷的時相.方法 建立單側輸尿管完全梗阻動物模型,梗阻後不同時間點取齣腎髒,經HE染色和免疫熒光雙標方法,觀察腎髒病理及足細胞錶型的改變.結果 大體觀察,梗阻24 h後齣現腎孟擴張,梗阻3 d後齣現腎皮質變薄.組織學觀察,梗阻12 h後齣現一部分近麯小管擴張,小管上皮細胞呈水樣變性腫脹,在梗阻3 d後齣現大量小管上皮細胞變形腫脹.免疫熒光雙標法,梗阻24 h後有少量足細胞、巨噬細胞同時被2種抗體標記,2 d後足細胞呈減少趨勢,而巨噬細胞逐漸增多.結論 輸尿管梗阻後在腎髒病理改變早于腎髒髮生形態學改變的髮生,輸尿管梗阻後2A h髮生足細胞錶型的改變,與腎髒腎盂齣現明顯擴張的時間相近,隨著梗阻時間的延長,腎髒病理改變逐漸加重,足細胞轉化逐漸增多.
목적 관찰서수뇨관완전경조후신장병리화족세포원형개변적시서변화,심조수뇨관경조후신장발생불가역손상적시상.방법 건립단측수뇨관완전경조동물모형,경조후불동시간점취출신장,경HE염색화면역형광쌍표방법,관찰신장병리급족세포표형적개변.결과 대체관찰,경조24 h후출현신맹확장,경조3 d후출현신피질변박.조직학관찰,경조12 h후출현일부분근곡소관확장,소관상피세포정수양변성종창,재경조3 d후출현대량소관상피세포변형종창.면역형광쌍표법,경조24 h후유소량족세포、거서세포동시피2충항체표기,2 d후족세포정감소추세,이거서세포축점증다.결론 수뇨관경조후재신장병리개변조우신장발생형태학개변적발생,수뇨관경조후2A h발생족세포표형적개변,여신장신우출현명현확장적시간상근,수착경조시간적연장,신장병리개변축점가중,족세포전화축점증다.
Objective To find time pint of the irreversible kidney injuru by observing renal pathological appearance and podocytes phenotype after complete ureteral obstruction.Methods The animal model of complete unilateral uretersal obstruction was consructed in 2-weekold and 2-month-old rats respectively.HE staining was employed to evaluate the degree of pathological changes,and immunofluorescence double staining to observe the transitional progression of podocyte.Results Grossly,there appeared pyelectasis at 2 h after obstruction and thinner renal cortex after 3 days of obstruction.Histologically,proximal tube display dilated and hydropic degeneration of tubular epithelial cells was observed after 12-day obstruction.The tubular epithelium showed distorted swelling after 3-day obstruction.Immunofluorescence double staining showed that podocytes decreased and the double-labeled macrophages was increased during 1 d to 2 d after unilateral ureteral obstruction operation.Conclusion Compared with control groups,the unilateral ureteral obstruction groups shows podocyte-to-macrophage transition at 1 d after the operation, while the pelvis shows obviously dilated.From the 1st day to the 7th day after unilateral ureteral obstruction,it shows increasing tendency of the podocytes transition.
