CAJ | 학술논문

背景:研究发现一些植物雌激素可改变生殖系统发育,并可能最终影响生殖衰老的年龄.槲皮素是一种黄酮类物质,具有微弱的雌激素效应,但对卵巢卵泡发育和卵巢衰老进程究竟有无影响还不清楚.目的:观察槲皮素对大鼠卵巢卵泡发育和卵巢寿命的影响.方法:健康成年SD大鼠30只雌雄按2:1合笼所生的新生雌鼠,分为3组:母鼠灌胃组,对孕10.5 d母鼠槲皮素灌胃,1次/d,直至分娩,取1,2,4 d新生雌鼠;腹腔注射组,对正常出生后12 h内新生雌鼠槲皮素腹腔注射,1次/d,取2,4 d新生雌鼠:空白对照组:不进行任何干预的1,2,4 d新生雌鼠;观察新生鼠卵巢发育情况.另取12月龄雌性大鼠22只,以数字表法随机分为实验组和对照组:实验组用槲皮素灌胃4个月/1次/d.对照组灌胃给予生理盐水.对灌胃前和灌胃期间12月龄雌性大鼠行阴道涂片,观察大鼠动情周期模式:灌胃结束后,计算卵巢和子宫系数,苏木精一伊红染色观察各期卵泡发育情况和卵泡总擞变化.结果与结论:用槲皮索干预1,2 d龄新生大鼠未装配的卵泡均减少,而发育更晚期阶段的卵泡即原始卵泡或发育卵泡有所增加.灌胃前大部分大鼠动情周期已经不规则,用药后,随着大鼠年龄的增长,实验组与对照组显示了相似的动情周期变化:规则的动情逐渐消失,周期变得延长或不规则,灌胃结束时均以不规则动情为主.灌胃4个月后,实验组大鼠卵巢大部分由闭锁卵泡组成,另外可见少许原始卵泡、发育卵泡以及窦状卵泡和黄体,实验组窦状卵泡较对照组增加(P<0.05),两组卵巢和子宫系数及体质量增加无差异.提示槲皮素可能具有一定促进卵巢卵泡发育和成熟的作用,但不影响卵巢寿命.
배경:연구발현일사식물자격소가개변생식계통발육,병가능최종영향생식쇠로적년령.곡피소시일충황동류물질,구유미약적자격소효응,단대란소란포발육화란소쇠로진정구경유무영향환불청초.목적:관찰곡피소대대서란소란포발육화란소수명적영향.방법:건강성년SD대서30지자웅안2:1합롱소생적신생자서,분위3조:모서관위조,대잉10.5 d모서곡피소관위,1차/d,직지분면,취1,2,4 d신생자서;복강주사조,대정상출생후12 h내신생자서곡피소복강주사,1차/d,취2,4 d신생자서:공백대조조:불진행임하간예적1,2,4 d신생자서;관찰신생서란소발육정황.령취12월령자성대서22지,이수자표법수궤분위실험조화대조조:실험조용곡피소관위4개월/1차/d.대조조관위급여생리염수.대관위전화관위기간12월령자성대서행음도도편,관찰대서동정주기모식:관위결속후,계산란소화자궁계수,소목정일이홍염색관찰각기란포발육정황화란포총수변화.결과여결론:용곡피색간예1,2 d령신생대서미장배적란포균감소,이발육경만기계단적란포즉원시란포혹발육란포유소증가.관위전대부분대서동정주기이경불규칙,용약후,수착대서년령적증장,실험조여대조조현시료상사적동정주기변화:규칙적동정축점소실,주기변득연장혹불규칙,관위결속시균이불규칙동정위주.관위4개월후,실험조대서란소대부분유폐쇄란포조성,령외가견소허원시란포、발육란포이급두상란포화황체,실험조두상란포교대조조증가(P<0.05),량조란소화자궁계수급체질량증가무차이.제시곡피소가능구유일정촉진란소란포발육화성숙적작용,단불영향란소수명.
BACKGROUND:Research demonstrated that some phytoestrogens can change the reproductive system development and may affect the age of reproductive senescence ultimately.Quercetin is a plant-derived fiavonoid,which possess weak estrogen effect,however,there is limited evidence that quercetin may has effect on ovadan fcllicular development or ovarian consenescence.0BJECTIVE:To explore the effects of quercetin on ovadan follicular development and ovadan life span in rats.METHOD:A total of 30 SD rats were caged together with male and female ratio of 2:1,and the neonatal female rates were divided into three groups.①Intragastric perfusion group,pregnant rats were intragastric administrated quercetin(40 mg/kg.d)on day 10.5 after pregnancy till delivery,and the female rats with 1 d,2 d and 4 d were selected;②Intraperitoneal injection group,female neonatal rats were intrapedtoneally injected with quercetin within 12 hours after birth,once per day,and 2 d and 4 d female rats were selected;③Blank control group,2 d and 4 d female rats were selected without intervention.The ovanan development of rats was observed.Additional 22 female rats were divided into the experimental and control groups according to the number table method.Rats in the experimental group were gastrogavage administered 40 mg/kg quercetin once per day for 4 consecutive months,and rats in the control group were fed with normal saline of same volume instead.Early aged rats were killed after completing administration,uerus and ovanes were weighed to calculate their coefficients and ovarian morphology were observed;In addition,vaginal smears were used to observe estrous cydes patterns.RESULTS AND CONCLUSION:The percentage of unassembled follicles was decreesed and primordial follicles increased in the 1/2-day-old rat's ovaries of intragastdc adrrrinistration group.The rats oestrus cycles were became irregular before intragastdc administration,which exhibited similar oestrus cycles in the experimental and control groups after medication.At month 4 after intragastric administration,the ovanes of rats in the experimental group comprised atretic follicle,primordial follicle,developed follicles.as well as atretic follicles and corpus luteum,and the number was greater than that of the control group(P<0.05),however,the coefficients of ovarian and uterine and increased body mass had no significant difierence between the experimental and control groups.The results demonstrated that quercetin may promote ovarian follicular development and matudty,but noeffect onthe ovanan life span.