CAJ | 학술논문

用小鼠和人的SRPK1基因的编码区序列通过NCBI数据库进行比较和检索,得到一个高同源性的猪cDNA序列和4个猪SRPK1基因的ESTs.利用DNAMAN软件将以上片段进行拼接,得到了一条较长的拼接片段X-1.ORF finder和Blast 2 sequence程序分析.结果表明,X-1中包含一个完整的长为1 968 bp的编码区,编码656个氨基酸,且此编码区DNA序列与人的SRPK1基因有91.73%的一致性,氨基酸序列的一致性为92.93%.
용소서화인적SRPK1기인적편마구서렬통과NCBI수거고진행비교화검색,득도일개고동원성적저cDNA서렬화4개저SRPK1기인적ESTs.이용DNAMAN연건장이상편단진행병접,득도료일조교장적병접편단X-1.ORF finder화Blast 2 sequence정서분석.결과표명,X-1중포함일개완정적장위1 968 bp적편마구,편마656개안기산,차차편마구DNA서렬여인적SRPK1기인유91.73%적일치성,안기산서렬적일치성위92.93%.
Use the SRPK1 sequence of human and mouse to compare and search in NCBI date, where a high similar cDNA sequence and four pigs' SRPK1 ESTs were found, which were spliced into a longer one (X-1) by DNAMAN software. A complete opening frame of 1 968 bp length, was found by ORF finder and Blast 2 sequence in the X-1 sequence, which encoded a protein of 656 amino acids. This coding sequence of 1pin-old showed 91.73% indentity in 1 968 bp segments with the human SRPK1 gene and had 92.93% identity in 656 amino acids.