国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2008年
2期
64-67
,共4页
环介导恒温扩增%寄生虫%检测%应用
環介導恆溫擴增%寄生蟲%檢測%應用
배개도항온확증%기생충%검측%응용
Loop-mediated isothermal amplification%Parasites%Detection%Application
近年来开发出一种新的恒温核酸扩增方法,即环介导恒温扩增(loop-mediated isithermal amplification,LAMP)法.LAMP技术具有简便、快速、高效、特异性强等优点,尤其适用于人类和动物疾病的检测.该文简要介绍了LAMP技术的原理,并对其在检测寄生虫如锥虫、疟原虫、巴贝虫、隐孢子虫和水生动物孢子虫等方面的应用进行综述.
近年來開髮齣一種新的恆溫覈痠擴增方法,即環介導恆溫擴增(loop-mediated isithermal amplification,LAMP)法.LAMP技術具有簡便、快速、高效、特異性彊等優點,尤其適用于人類和動物疾病的檢測.該文簡要介紹瞭LAMP技術的原理,併對其在檢測寄生蟲如錐蟲、瘧原蟲、巴貝蟲、隱孢子蟲和水生動物孢子蟲等方麵的應用進行綜述.
근년래개발출일충신적항온핵산확증방법,즉배개도항온확증(loop-mediated isithermal amplification,LAMP)법.LAMP기술구유간편、쾌속、고효、특이성강등우점,우기괄용우인류화동물질병적검측.해문간요개소료LAMP기술적원리,병대기재검측기생충여추충、학원충、파패충、은포자충화수생동물포자충등방면적응용진행종술.
In recent years,a new isothermal nucleic acid amplification method,100p-mediated isothermal amplification (LAMP)method has been developed and applied.LAMP is a simple,rapid,highlv efficient,highly specific gene amplification method,particularly suitable for human and animal disease detection.This paper briefly summarized the principal of LAMP and its applications in parasites detection,such as Trypanosoma,Plusmodium,Babesia,Cryptosporidium and Tetracapsuloides bryosalmonae in aquatic animals.
