CAJ | 학술논문

目的探讨急性肺损伤(ALI)时血中中性粒细胞弹性蛋白酶(NE)、肺组织肺表面活性物质蛋白A(SP-A)的变化及抗氧化剂的影响效应.方法健康、成熟Wistar大鼠60只随机均分为2组,模型组和治疗组,2组均以大肠杆菌腹腔注射建立ALI动物模型.治疗组在注射大肠杆菌30 min后,经大鼠尾静脉注射还原型谷胱甘肽.分别于注射大肠杆菌后3、6、12 h测定血中NE及肺组织SP-A的变化.结果模型组注射大肠杆菌后3 h出现ALI症状,6 h更为明显,12 h口吐粉红色分泌物.肺组织大体病理检查可见双肺有明显水肿及出血点.治疗组3h无明显变化,6h呼吸稍促,12h肺组织仅轻度水肿.治疗组3、6、12 h测得血中NE水平低于模型组,在6、12 h差异有显著性(P<0.05);3、6、12 h测得肺组织SP-A阳性表达细胞数均高于模型组,差异均有显著性(P<0.05).结论 ALI时肺表面活性物质功能失常是继发性变化,其中原因之一就是SP-A被降解,应用抗氧化剂--还原型谷胱甘肽后可有效抑制NE分解细胞外基层弹性蛋白、破坏表面活性蛋白的作用,对ALI具有保护效应.
목적 탐토급성폐손상(ALI)시혈중중성립세포탄성단백매(NE)、폐조직폐표면활성물질단백A(SP-A)적변화급항양화제적영향효응.방법 건강、성숙Wistar대서60지수궤균분위2조,모형조화치료조,2조균이대장간균복강주사건립ALI동물모형.치료조재주사대장간균30 min후,경대서미정맥주사환원형곡광감태.분별우주사대장간균후3、6、12 h측정혈중NE급폐조직SP-A적변화.결과 모형조주사대장간균후3 h출현ALI증상,6 h경위명현,12 h구토분홍색분비물.폐조직대체병리검사가견쌍폐유명현수종급출혈점.치료조3h무명현변화,6h호흡초촉,12h폐조직부경도수종.치료조3、6、12 h측득혈중NE수평저우모형조,재6、12 h차이유현저성(P<0.05);3、6、12 h측득폐조직SP-A양성표체세포수균고우모형조,차이균유현저성(P<0.05).결론 ALI시폐표면활성물질공능실상시계발성변화,기중원인지일취시SP-A피강해,응용항양화제--환원형곡광감태후가유효억제NE분해세포외기층탄성단백、파배표면활성단백적작용,대ALI구유보호효응.
Objective To explore the changes of neutrophil elastase (NE) and surfactant protein A (SP-A) in acute lung injury(ALI) rats,and the effect of antioxidant. Methods Sixty healthy mature Wister rats were divided into 2 groups, the control group and treatment group. The rats in two groups all received peritoneal injection of E. coli to establish the ALI animal model. 30 minutes after injection of E. coli,the rats in treatment group were injected reduced glutathione from vena caudalis. The levels of NE in blood and expressions of SP-A in lung tissue were detected at 3,6 and 12 hours after injection of E. coli. Results ALI symptom appeared 3 hours after injection of E. coli in the control group, obvious after 6 hours, the rats vomi-ted pink secretion after 12 hours. Lung edema and bleeding were found by pathologic examination. No obvious symptom was found in treatment group after 3 hours, slight tachypnea after 6 hours, slight edema in pulmonary tissue after 12 hours. After administration of reduced glutathione,levels of NE at 3,6 and 12 hours in the treatment group were lower than those in the control group,and indicated statistical significance in 6 and 12 hours(P <0. 05) ;Levels of SP-A in 3,6 and 12 hours in the treatment group were higher than those in the control group, and indicated statistical significance in 3,6 and 12 hours (P < 0. 05). Conclusion Dysfunction of pulmonary surfactant is secondary in ALI, degradation of SP-A is the one of reasons, the application of reduced glutathione as antioxidant, could effectively suppress NE to decompose basosexine elastin of cells and destroy surface active protein, has protective effect on ALI.