中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2009年
5期
308-310
,共3页
疱疹,生殖器%杀伤细胞,天然%受体%共刺激分子
皰疹,生殖器%殺傷細胞,天然%受體%共刺激分子
포진,생식기%살상세포,천연%수체%공자격분자
Herpes genitalis%Killer cells,natural%Receptors%Costimulatory molecules
目的 探讨NK细胞表面激活性受体(NKG2D、NKp46)和抑制性受体(NKG2A、KIR)以及共刺激分子(OX40、4-1BB、ICOS)的表达在复发性生殖器疱疹(RGH)发病中的作用.方法 采用四色免疫荧光抗体染色,经流式细胞仪检测44例RGH患者外周血NK细胞表达NKG2D、NKp46、NKG2A、K1R的水平及29例RGH患者NK细胞上OX40、4-1BB、ICOS分子表达.结果 RGH患者外周血NK细胞表达激活性受体NKG2D和NKp46的百分比分别为(93.3±5.4)%和(88.9±8.7)%,均显著低于对照组的(96.9±2.5)%和(93.4±4.1)%,P值分别<0.01.抑制性受体NKG2A、KIR的表达水平差异均无统计学意义(P>0.05)、共刺激分子OX40表达下调,患者和对照组分别为(1.0±1.1)%和(1.8±1.7)%(P<0.05).结论 单纯疱疹病毒感染能下调NK细胞激活性受体及共刺激分子表达,抑制NK细胞的活化,使病毒感染细胞逃逸NK细胞的天然杀伤作用.
目的 探討NK細胞錶麵激活性受體(NKG2D、NKp46)和抑製性受體(NKG2A、KIR)以及共刺激分子(OX40、4-1BB、ICOS)的錶達在複髮性生殖器皰疹(RGH)髮病中的作用.方法 採用四色免疫熒光抗體染色,經流式細胞儀檢測44例RGH患者外週血NK細胞錶達NKG2D、NKp46、NKG2A、K1R的水平及29例RGH患者NK細胞上OX40、4-1BB、ICOS分子錶達.結果 RGH患者外週血NK細胞錶達激活性受體NKG2D和NKp46的百分比分彆為(93.3±5.4)%和(88.9±8.7)%,均顯著低于對照組的(96.9±2.5)%和(93.4±4.1)%,P值分彆<0.01.抑製性受體NKG2A、KIR的錶達水平差異均無統計學意義(P>0.05)、共刺激分子OX40錶達下調,患者和對照組分彆為(1.0±1.1)%和(1.8±1.7)%(P<0.05).結論 單純皰疹病毒感染能下調NK細胞激活性受體及共刺激分子錶達,抑製NK細胞的活化,使病毒感染細胞逃逸NK細胞的天然殺傷作用.
목적 탐토NK세포표면격활성수체(NKG2D、NKp46)화억제성수체(NKG2A、KIR)이급공자격분자(OX40、4-1BB、ICOS)적표체재복발성생식기포진(RGH)발병중적작용.방법 채용사색면역형광항체염색,경류식세포의검측44례RGH환자외주혈NK세포표체NKG2D、NKp46、NKG2A、K1R적수평급29례RGH환자NK세포상OX40、4-1BB、ICOS분자표체.결과 RGH환자외주혈NK세포표체격활성수체NKG2D화NKp46적백분비분별위(93.3±5.4)%화(88.9±8.7)%,균현저저우대조조적(96.9±2.5)%화(93.4±4.1)%,P치분별<0.01.억제성수체NKG2A、KIR적표체수평차이균무통계학의의(P>0.05)、공자격분자OX40표체하조,환자화대조조분별위(1.0±1.1)%화(1.8±1.7)%(P<0.05).결론 단순포진병독감염능하조NK세포격활성수체급공자격분자표체,억제NK세포적활화,사병독감염세포도일NK세포적천연살상작용.
Objective To investigate the expression of activating receptors (NKG2D and NKp46), inhibitory receptors (NKG2A and KIR) as well as costimulatory molecules (OX40, 4-1BB and ICOS) on peripheral blood natural killer (NK) cells from patients with recurrent genital herpes (RGH). Methods Four-color immunofluorescence staining with flow cytometry was used to detect the expression of NKG2D, NKG2A, KIR and NKp46 in 44 patients with RGH and 40 normal human controls, and to detect the expression of OX40, 4-1BB and ICOS in 29 patients with RGH and 29 normal human controls. Results The proportions of NKG2D-positive and NKp46-positive NK cells significantly decreased in patients with RGH than those in the normal human controls [(93.3 ± 5.4)% vs (96.9 ± 2.5)%, (88.9 ± 8.7)% vs(93.4 ± 4.1)%, respectively, both P < 0.01]. Between the patients and controls, no significant difference was observed in the expression of NK cell inhibitory receptors, NKG2A [(41.8 ± 14.4)% vs (46.0 ± 14.7)%, P > 0.05] or KIR [(68.3 ± 19.1)% vs (69.1 ± 17.6)%, P > 0.05]. A lower expression of costimulatory molecule OX40 was noted in NK cells from patients with RGH compared with those in normal controls [(1.0 ± 1.1)% vs (1.8 ± 1.7)%, P < 0.05]. Conclusions Herpes simplex virus infection could down-regulate the expression of NK cell activating receptors and costimulatory molecules, subsequently suppress the activation of NK cells, and lead to the escape of virus-infected cells from the killing of NK cells.
