CAJ | 학술논문

目的联合三聚氰胺与三聚氰酸灌胃造成大鼠急性肾损伤,观察利尿剂呋塞米的治疗作用.方法将36只成年雄性SD大鼠随机平均分为3组,分别为A组(正常对照组),灌胃2 ml蒸馏水,B组(模型组),灌胃三聚氰胺(100 mg/kg)与三聚氰酸(100 mg/kg),连续4 d,4 d后改为灌胃2ml蒸馏水,C组(治疗组):前4 d与B组一样,4 d后为呋塞米(20 mg/kg);第4天、第11天后各处死6只大鼠,观察大鼠肾脏病理,肾内结晶沉积评分情况、大鼠血和尿多项生化指标的变化.结果在第4天时,B组和C组分别出现大鼠少尿[(3.39±1.02)ml、(3.20±0.86)ml]、高血肌酐[(153.54±27.08)μmol/L、(160.11±19.55)μmol/L]等急性肾损伤表现,并在肾内观察到三聚氰胺氰尿酸盐晶体.第11天与第4天时肾内结晶评分相比,B组减少了9.52%(P>0.05).C组减少了63.63%(P<0.05),第11天时,B组尿量[(8.57±1.66)m1]和C组尿量[(25.96±5.97)ml]分别较第4天时尿量[(3.39±1.02)ml、(3.20±0.86)ml]显著增加(P<0.05),其中C组[(25.96±5.97)ml]较B组[(8.57±1.66)ml]增加更明显(P<0.05);B组和C组血清肌酐[(106.10±5.53)μmol/L、(67.17±12.80)μmol/L]分别较第4天时[(153.54±27.08)μmol/L、(160.11±19.55)μmol/L]显著减低(P<0.05),但B组高于A组和C组(P<0.05).结论呋塞米对三聚氰胺与三聚氰酸所致急性肾损伤起一定保护作用.
목적 연합삼취청알여삼취청산관위조성대서급성신손상,관찰이뇨제부새미적치료작용.방법 장36지성년웅성SD대서수궤평균분위3조,분별위A조(정상대조조),관위2 ml증류수,B조(모형조),관위삼취청알(100 mg/kg)여삼취청산(100 mg/kg),련속4 d,4 d후개위관위2ml증류수,C조(치료조):전4 d여B조일양,4 d후위부새미(20 mg/kg);제4천、제11천후각처사6지대서,관찰대서신장병리,신내결정침적평분정황、대서혈화뇨다항생화지표적변화.결과 재제4천시,B조화C조분별출현대서소뇨[(3.39±1.02)ml、(3.20±0.86)ml]、고혈기항[(153.54±27.08)μmol/L、(160.11±19.55)μmol/L]등급성신손상표현,병재신내관찰도삼취청알청뇨산염정체.제11천여제4천시신내결정평분상비,B조감소료9.52%(P>0.05).C조감소료63.63%(P<0.05),제11천시,B조뇨량[(8.57±1.66)m1]화C조뇨량[(25.96±5.97)ml]분별교제4천시뇨량[(3.39±1.02)ml、(3.20±0.86)ml]현저증가(P<0.05),기중C조[(25.96±5.97)ml]교B조[(8.57±1.66)ml]증가경명현(P<0.05);B조화C조혈청기항[(106.10±5.53)μmol/L、(67.17±12.80)μmol/L]분별교제4천시[(153.54±27.08)μmol/L、(160.11±19.55)μmol/L]현저감저(P<0.05),단B조고우A조화C조(P<0.05).결론 부새미대삼취청알여삼취청산소치급성신손상기일정보호작용.
Objective To investigate the effect of diuretic (furosemide) therapy on kidney injury induced by melamine and cyanuric acid in rats. Methods 36 male Spragne Dawley rats were random disided into 3 groups. Group A was treated with 2mL of water daily, group B was treated with melamine and cyanuric acid ( each 100 mg/kg) daily for 4 days and then 2ml of water daily, group C was treated with the same as group B at the first 4 days and then treatment with furosemide (20mg/kg) daily. Samples of blood and 24h urine were collected to detective biochemical indexes, and kidney sections were performed on days 4 and 11 ( each end point, n = 6). The kidneys were observed with histopathology and renal crystal deposition scores were determined. Results On the 4th day, group B and group C were resulted in acute kidney injury such as oliguria [ ( 3. 39 ± 1.02 ) ml, ( 3. 20 ± 0. 86 ) ml ] and high serum creatinine [ ( 153.54 ±27. 08)μmol/L, (160. 11 ± 19. 55)μmol/L] and renal melamine cyanurate crystal were found in the renal tissues. On the 11th day, the renal crystal deposition score in the rats was reduced by 9. 52% ( P >0. 05). Compared with those of the 4th day in group B, it reduced by 63.63%( P <0.05) in group C. Urine volume were increased significantly compared with those of the 4th day( P < 0. 05 ) in group C [ from (3.20±0. 86)ml to (25.96 ±5.97)ml] and group B [ from(3. 39 ± 1.02)ml to (8. 57 ± 1.66)ml] , and Urine volume in group C was increased significantly more than that in group B ( P < 0. 05 ). The serum creatinine was obviously reduced as compared with those of the 4th day in group B and C( P <0.05), from[ (153. 54±27.08) μmol/L] to [ ( 106. 10 ±5.53) μmol/L] in group B and from [ ( 160. 11 ± 19. 55) μmol/L] to [ (67. 17 ± 12. 80 ) μmol/L] in group C, but the serum creatinine in group B was still higher than that in group A and C ( P < 0. 05). Conclusions Furosemide can attenuate the damage of acute kidney injury induced by melamine and cyanuric acid.