中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2011年
11期
1441-1444
,共4页
蒋艳萍%洛若愚%陈朗%曹来英
蔣豔萍%洛若愚%陳朗%曹來英
장염평%락약우%진랑%조래영
子宫内膜异位症/治疗/代谢%抗原,CD19/代谢%抗原,CD5/代谢%B淋巴细胞/ME%干扰素Ⅱ型/代谢%白细胞介素10/代谢
子宮內膜異位癥/治療/代謝%抗原,CD19/代謝%抗原,CD5/代謝%B淋巴細胞/ME%榦擾素Ⅱ型/代謝%白細胞介素10/代謝
자궁내막이위증/치료/대사%항원,CD19/대사%항원,CD5/대사%B림파세포/ME%간우소Ⅱ형/대사%백세포개소10/대사
Endometriosis/TH/ME%Antigens,CD 19/ME%Antigens,CD5/ME%B-lymphocytes/ME%Interferon type Ⅱ/ME%Interleukin-10/ME
目的 研究子宫内膜异位症患者治疗前、后CD19+ CD5+B细胞数量及分泌细胞因子的变化,为临床治疗提供实验依据.方法 对本院35例子宫内膜异位症患者(实验组),29例子宫肌瘤患者(对照组)行流式细胞术、Q-PCR检测患者外周血、腹腔冲洗液及子宫内膜组织中CD19+CD5+B细胞的数量及分泌细胞因子的检测,比较治疗前、后子宫内膜异位症患者外周血中CD19+CD5+B细胞数量以及分泌细胞因子的变化.结果 子宫内膜异位症患者外周血、腹腔冲洗液及异位子宫内膜组织中CD19+ CD5+B细胞的比例高于对照组[(13.1±1.9)％、(12.1±2.0)％、(11.7±1.7)％vs(2.9±0.8)％、(2.6±0.9)％、(2.8±1.1)％,P ＜0.01],CD19+ CD5+B细胞的IFN-γ mRNA的表达水平高于对照组[(7.2±1.0) ×103、(7.9±1.3) ×103、(7.4±1.1)×103拷贝vs (1.9±0.7)×103、(2.2±0.8) ×103、(2.0±0.5) ×103拷贝],IL-10 mRNA表达水平高于对照组[(6.4±0.9)×103、(6.8±1.2) ×103、(6.1±0.8)×103拷贝vs (1.7±0.5) ×103、(2.1±0.9)×103、(1.6±0.4)×103拷贝,P ＜0.01].治疗后,子宫内膜异位症患者外周血中CD19+ CD5+B细胞数量低于治疗前[(13.1±1.9)％vs(7.3±1.2)％,t=16.12,P＜0.01],仍高于对照组[(2.8±0.7)％,t=18.51,P＜0.01],CD19+ CD5+B细胞中的IFN-γ mRNA的表达水平低于治疗前[(7.2±1.0)×103拷贝vs (3.3±0.6)×103拷贝,t=20.89,P＜0.01],仍高于对照组[(2.4±0.5)×103拷贝,t=6.702,P＜0.01],IL-10 mRNA的表达水平低于治疗前[(6.4±0.9)×103拷贝vs (3.2±0.7)×103拷贝,t=17.53,P＜0.01],仍高于对照组[(2.1±0.4)×103拷贝,t =7.79,P＜0.01].结论 子宫内膜异位症中CD19+ CD5+B细胞数量的增加,通过高分泌IFN-γ、IL-10,其参与了疾病的发生.
目的 研究子宮內膜異位癥患者治療前、後CD19+ CD5+B細胞數量及分泌細胞因子的變化,為臨床治療提供實驗依據.方法 對本院35例子宮內膜異位癥患者(實驗組),29例子宮肌瘤患者(對照組)行流式細胞術、Q-PCR檢測患者外週血、腹腔遲洗液及子宮內膜組織中CD19+CD5+B細胞的數量及分泌細胞因子的檢測,比較治療前、後子宮內膜異位癥患者外週血中CD19+CD5+B細胞數量以及分泌細胞因子的變化.結果 子宮內膜異位癥患者外週血、腹腔遲洗液及異位子宮內膜組織中CD19+ CD5+B細胞的比例高于對照組[(13.1±1.9)％、(12.1±2.0)％、(11.7±1.7)％vs(2.9±0.8)％、(2.6±0.9)％、(2.8±1.1)％,P ＜0.01],CD19+ CD5+B細胞的IFN-γ mRNA的錶達水平高于對照組[(7.2±1.0) ×103、(7.9±1.3) ×103、(7.4±1.1)×103拷貝vs (1.9±0.7)×103、(2.2±0.8) ×103、(2.0±0.5) ×103拷貝],IL-10 mRNA錶達水平高于對照組[(6.4±0.9)×103、(6.8±1.2) ×103、(6.1±0.8)×103拷貝vs (1.7±0.5) ×103、(2.1±0.9)×103、(1.6±0.4)×103拷貝,P ＜0.01].治療後,子宮內膜異位癥患者外週血中CD19+ CD5+B細胞數量低于治療前[(13.1±1.9)％vs(7.3±1.2)％,t=16.12,P＜0.01],仍高于對照組[(2.8±0.7)％,t=18.51,P＜0.01],CD19+ CD5+B細胞中的IFN-γ mRNA的錶達水平低于治療前[(7.2±1.0)×103拷貝vs (3.3±0.6)×103拷貝,t=20.89,P＜0.01],仍高于對照組[(2.4±0.5)×103拷貝,t=6.702,P＜0.01],IL-10 mRNA的錶達水平低于治療前[(6.4±0.9)×103拷貝vs (3.2±0.7)×103拷貝,t=17.53,P＜0.01],仍高于對照組[(2.1±0.4)×103拷貝,t =7.79,P＜0.01].結論 子宮內膜異位癥中CD19+ CD5+B細胞數量的增加,通過高分泌IFN-γ、IL-10,其參與瞭疾病的髮生.
목적 연구자궁내막이위증환자치료전、후CD19+ CD5+B세포수량급분비세포인자적변화,위림상치료제공실험의거.방법 대본원35례자궁내막이위증환자(실험조),29례자궁기류환자(대조조)행류식세포술、Q-PCR검측환자외주혈、복강충세액급자궁내막조직중CD19+CD5+B세포적수량급분비세포인자적검측,비교치료전、후자궁내막이위증환자외주혈중CD19+CD5+B세포수량이급분비세포인자적변화.결과 자궁내막이위증환자외주혈、복강충세액급이위자궁내막조직중CD19+ CD5+B세포적비례고우대조조[(13.1±1.9)％、(12.1±2.0)％、(11.7±1.7)％vs(2.9±0.8)％、(2.6±0.9)％、(2.8±1.1)％,P ＜0.01],CD19+ CD5+B세포적IFN-γ mRNA적표체수평고우대조조[(7.2±1.0) ×103、(7.9±1.3) ×103、(7.4±1.1)×103고패vs (1.9±0.7)×103、(2.2±0.8) ×103、(2.0±0.5) ×103고패],IL-10 mRNA표체수평고우대조조[(6.4±0.9)×103、(6.8±1.2) ×103、(6.1±0.8)×103고패vs (1.7±0.5) ×103、(2.1±0.9)×103、(1.6±0.4)×103고패,P ＜0.01].치료후,자궁내막이위증환자외주혈중CD19+ CD5+B세포수량저우치료전[(13.1±1.9)％vs(7.3±1.2)％,t=16.12,P＜0.01],잉고우대조조[(2.8±0.7)％,t=18.51,P＜0.01],CD19+ CD5+B세포중적IFN-γ mRNA적표체수평저우치료전[(7.2±1.0)×103고패vs (3.3±0.6)×103고패,t=20.89,P＜0.01],잉고우대조조[(2.4±0.5)×103고패,t=6.702,P＜0.01],IL-10 mRNA적표체수평저우치료전[(6.4±0.9)×103고패vs (3.2±0.7)×103고패,t=17.53,P＜0.01],잉고우대조조[(2.1±0.4)×103고패,t =7.79,P＜0.01].결론 자궁내막이위증중CD19+ CD5+B세포수량적증가,통과고분비IFN-γ、IL-10,기삼여료질병적발생.
Objective To explore the changes of the frequency and cytokine production of CD19 + CD5 + B cells in Endometriosis before and after treatment.Methods Flow cytometry and quantitative PCR were used to assess the frequency,cytokine expression of CD19 + CD5 + B cells in the peripheral blood,peritoneal fluids and endometrium biopsies of 35 patients with Endometriosis and 29 patients with myoma of uterus (control subjects).Changes in the CD19+ CD5+ B cell compartment in the peripheral blood were compared before and after treatment.Results All 35 endometriosis patients had higher frequencies(all P ＜0.01 ) of CD19+ CD5 + B cells in the peripheral blood,peritoneal fluids and endometrium biopsies [ (13.1 ± 1.9)％,(12.1 ±2.0)％,(11.7 ± 1.7)％] than 29 control subjects[ (2.9 ±0.8)％,(2.6 ±0.9)％,(2.8 ±1.1 )％ ].CD19 + CD5+ B cells from endometriosis patients expressed higher IFN-γ[ (7.2 ± 1.0) × 103,(7.9±1.3) ×103,(7.4±1.1) ×103 copies] than control subjects [ (1.9±0.7) ×103,(2.2±0.8) ×103,(2.0 ±0.5) × 103 copies).The endometriosis patients also had higher IL-10 production of CD19 + CD5 + B cells [ (6.4 ±0.9) × 103,(6.8 ± 1.2) × 103,(6.1 ±0.8) × 103 copies] than control subjects [ ( 1.7 ±0.5) × 103,(2.1 ±0.9) × 103,( 1.6 ±0.4) × 103 copies].Compared with control subjects,the endometriosis patients had a clinical response to treatment demomtrated a significant decrease in CD19 + CD5 + B cells in the peripheral blood[ ( 13.1 ± 1.9)％ vs (7.3 ± 1.2)％ ],expressed lower IFN-γ [ (7.2 ± 1.0)×103 copies vs (3.3 ±0.6) × 103 copies],produced less IL-10 [(6.4 ±0.9) × 103 copies vs (3.2 ±0.7) × 103 copies].Conclusions CD19+ CD5 + B cells might play an important role in the pathogenesis of endometriosis through higher IFN-γand IL-10 expression.
