CAJ | 학술논문

目的探讨WWOX基因在肝细胞癌(HCC)和切缘中的表达状况和术后复发及预后的关系及其临床意义.方法用免疫组化方法检测76例肝癌组织及其切缘组织、20例正常肝组织中WWOX基因蛋白产物表达水平,用TUNEL法检测76例肝细胞癌的细胞凋亡水平,结合临床病理指标和术后生存率进行分析.结果 WWOX基因蛋白产物在HCC中定位于细胞核,在癌旁组织中定位于细胞核与细胞浆.肝癌组织中WWOX基因蛋白表达指数为(35.2±20.1)%,低于癌旁组织和正常肝组织(P<0.05).并与肝癌的细胞凋亡率下降、PCNA增高、肿瘤较大、门脉分支浸润、低分化高恶性度以及术后复发等指标有关(P<0.05).肝癌切缘ww0X基因蛋白表达水平与术后复发率和生存率有关(P<0.05),而与1 cm以上切缘距离无关.结论肝癌组织和手术切缘存在低WWOX表达和定位异常,并可能与影响肿瘤进展、预后的生物学行为有关.肝癌组织和手术切缘WWOX表达水平测定有望成为评估肝癌进展等生物学行为和预后的有用指标之一,并有助于确定较为安全的根治切除分子切缘.
목적 탐토WWOX기인재간세포암(HCC)화절연중적표체상황화술후복발급예후적관계급기림상의의.방법 용면역조화방법검측76례간암조직급기절연조직、20례정상간조직중WWOX기인단백산물표체수평,용TUNEL법검측76례간세포암적세포조망수평,결합림상병리지표화술후생존솔진행분석.결과 WWOX기인단백산물재HCC중정위우세포핵,재암방조직중정위우세포핵여세포장.간암조직중WWOX기인단백표체지수위(35.2±20.1)%,저우암방조직화정상간조직(P<0.05).병여간암적세포조망솔하강、PCNA증고、종류교대、문맥분지침윤、저분화고악성도이급술후복발등지표유관(P<0.05).간암절연ww0X기인단백표체수평여술후복발솔화생존솔유관(P<0.05),이여1 cm이상절연거리무관.결론 간암조직화수술절연존재저WWOX표체화정위이상,병가능여영향종류진전、예후적생물학행위유관.간암조직화수술절연WWOX표체수평측정유망성위평고간암진전등생물학행위화예후적유용지표지일,병유조우학정교위안전적근치절제분자절연.
Objective To investigate the clinical significance of WWOX expression in hepatocellular carcinoma(HCC) and resection margin and explore its relation to postoperative recurrence and prognosis.Methods The expression of WWOX protein was detected with immunohistochemical method-SP in 76 cases of HCC and resection margin and 20 cases of normal liver tissues,while the apoptosis rate was detected with TUNEL method in 76 cases of HCC.The relationship between these data and clinicopathological features was analyzed with SPSS software.Results Abnormal nuclear localization of WWOX protein in HCC and nuclear/ cytoplasm localization in resection margin were detected.The WWOXLI in HCC was 35.2 %±20.1%,lower than those of resection margin and normal liver tissues (P<0.05).The lower level of WWOX expression in HCC was significantly related to the lower apoptosis rate,higher PCNALI,huge tumor,cancerous thrombus in branches of portal vein,lower differentiation,lower survival rate and postoperative recurrence etc.(P<0.05).The WWOX expression level in resection margin was significantly related to postoperative recurrence rate(P<0.05) but not the distance of resection margin.Conclusion The lower expression and abnormal cellular location of WWOX gene in HCC can be a frequent event,which can be related to some biological characteristics of HCC to influence its progress and prognosis.The expression level of WWOX gene in HCC and resection margin may be one of useful indicators in forecasting the progress and prognosis and be used as a safe biomarker of molecular margin.