中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2012年
7期
526-529
,共4页
宫颈肿瘤%HeLa细胞%肿瘤侵润%ATP结合匣式转运子%肿瘤干细胞%肿瘤标记,生物学
宮頸腫瘤%HeLa細胞%腫瘤侵潤%ATP結閤匣式轉運子%腫瘤榦細胞%腫瘤標記,生物學
궁경종류%HeLa세포%종류침윤%ATP결합갑식전운자%종류간세포%종류표기,생물학
Uterine cervical neoplasms%HeLa cells%Neoplasm invasiveness%ATP-binding cassette transporters%Neoplastic stem cells%Tumor markers,biological
目的 通过对宫颈癌细胞系HeLa细胞中Bcrp1+表型细胞的侵袭和成瘤能力的研究,探讨Bcrp1成为宫颈癌干细胞表面标志物的可能性.方法 根据实验中所用HeLa细胞表型的不同分为两组,即Bcrp1+组和Bcrp1 -组,利用穿膜小室——boydon小室体外侵袭实验检测两组细胞的侵袭能力；将不同细胞密度梯度(1×104、1×105、1×106个/ml)的Bcrp1+及Bcrp1-组细胞分别接种于6～8周龄非糖尿病-严重免疫缺陷(NOD/SCID)小鼠体内,比较两组小鼠的体内成瘤情况,包括成瘤时间、成瘤率、肿瘤体积及肿瘤质量.结果 体外侵袭实验显示,Bcrp1+组穿膜细胞数为(99± 14)个,侵袭深度为(366±52) μm,明显高于Bcrp1-组的(57±13)个和(301±54) μm,两组分别比较,差异均有统计学意义(P＜0.05).体内成瘤实验显示,接种细胞密度为1 ×104个/ml时,Bcrp1+ 组小鼠体内即可形成移植瘤；接种细胞密度为1×105和1×106个/ml时,Bcrp1+组和Bcrp1-组小鼠体内均可形成移植瘤；与Bcrp1-组小鼠比较,Bcrp1+组小鼠接种相应细胞密度的成瘤时间早、成瘤率高、肿瘤体积和肿瘤质量大,两组分别比较,差异均有统计学意义(P＜0.05).结论 Bcrp1+表型的HeLa细胞与Bcrp1-表型相比,具有较强的成瘤及侵袭能力,具备肿瘤干细胞的部分特征,其中可能富集了宫颈癌干细胞.
目的 通過對宮頸癌細胞繫HeLa細胞中Bcrp1+錶型細胞的侵襲和成瘤能力的研究,探討Bcrp1成為宮頸癌榦細胞錶麵標誌物的可能性.方法 根據實驗中所用HeLa細胞錶型的不同分為兩組,即Bcrp1+組和Bcrp1 -組,利用穿膜小室——boydon小室體外侵襲實驗檢測兩組細胞的侵襲能力；將不同細胞密度梯度(1×104、1×105、1×106箇/ml)的Bcrp1+及Bcrp1-組細胞分彆接種于6～8週齡非糖尿病-嚴重免疫缺陷(NOD/SCID)小鼠體內,比較兩組小鼠的體內成瘤情況,包括成瘤時間、成瘤率、腫瘤體積及腫瘤質量.結果 體外侵襲實驗顯示,Bcrp1+組穿膜細胞數為(99± 14)箇,侵襲深度為(366±52) μm,明顯高于Bcrp1-組的(57±13)箇和(301±54) μm,兩組分彆比較,差異均有統計學意義(P＜0.05).體內成瘤實驗顯示,接種細胞密度為1 ×104箇/ml時,Bcrp1+ 組小鼠體內即可形成移植瘤；接種細胞密度為1×105和1×106箇/ml時,Bcrp1+組和Bcrp1-組小鼠體內均可形成移植瘤；與Bcrp1-組小鼠比較,Bcrp1+組小鼠接種相應細胞密度的成瘤時間早、成瘤率高、腫瘤體積和腫瘤質量大,兩組分彆比較,差異均有統計學意義(P＜0.05).結論 Bcrp1+錶型的HeLa細胞與Bcrp1-錶型相比,具有較彊的成瘤及侵襲能力,具備腫瘤榦細胞的部分特徵,其中可能富集瞭宮頸癌榦細胞.
목적 통과대궁경암세포계HeLa세포중Bcrp1+표형세포적침습화성류능력적연구,탐토Bcrp1성위궁경암간세포표면표지물적가능성.방법 근거실험중소용HeLa세포표형적불동분위량조,즉Bcrp1+조화Bcrp1 -조,이용천막소실——boydon소실체외침습실험검측량조세포적침습능력；장불동세포밀도제도(1×104、1×105、1×106개/ml)적Bcrp1+급Bcrp1-조세포분별접충우6～8주령비당뇨병-엄중면역결함(NOD/SCID)소서체내,비교량조소서적체내성류정황,포괄성류시간、성류솔、종류체적급종류질량.결과 체외침습실험현시,Bcrp1+조천막세포수위(99± 14)개,침습심도위(366±52) μm,명현고우Bcrp1-조적(57±13)개화(301±54) μm,량조분별비교,차이균유통계학의의(P＜0.05).체내성류실험현시,접충세포밀도위1 ×104개/ml시,Bcrp1+ 조소서체내즉가형성이식류；접충세포밀도위1×105화1×106개/ml시,Bcrp1+조화Bcrp1-조소서체내균가형성이식류；여Bcrp1-조소서비교,Bcrp1+조소서접충상응세포밀도적성류시간조、성류솔고、종류체적화종류질량대,량조분별비교,차이균유통계학의의(P＜0.05).결론 Bcrp1+표형적HeLa세포여Bcrp1-표형상비,구유교강적성류급침습능력,구비종류간세포적부분특정,기중가능부집료궁경암간세포.
Objective To make sure whether or not Bcrp1 is the marker of cervical cancer stem cells or not by studying the invasive ability and formation of tumors of Bcrp1 + phenotype HeLa cells.Methods The tumor cell migration and invasion assay were used by boyden chamber to identify the invasive ability of Bcrp1 + phenotype HeLa cells.The formation of tumors in vivo experiments were completed,in which the two groups of cells with different concentrations were inoculated in non obese diabetes-severe combined immunodeficiency disease ( NOD/SCID ) mice ( 1 × 104,1 × 105,1 × 106/ml ) and the differences of time,rate and volume in the formation of tumors between two groups were observed.Results ( 1 ) In the invasion assay,the amount of cells that invaded through the artificial basement membrane in Bcrp1 + group were 99 ± 14,which was significantly greater than those in Bcrp1- group ( 57 ± 13,P ＜ 0.05 ) ; the length of the Bcrp1 + group was ( 366 ± 52 ) μm,which was significantly greater than the Bcrp1 - group ( 301 ± 54) μm ( P ＜ 0.05 ).( 2 ) Following transplantation of 1 × 104 cells,only the Bcrp1 + cells formed tumors in NOD/SCID mice.When 1 × 105 or 1 × 106 cells were transplanted,the tumor incidence and the tumor mass were greater in the Bcrp1 + groups than those in the Bcrp1 - groups ( P ＜ 0.05 ).Conclusion Bcrp1 + HeLa cell have the greater capacity of invasive and the tumorigenicity,which may contain cancer stem cells.
