CAJ | 학술논문

目的探讨在不同刺激条件下,胸腺五肽(TP5)对重症肌无力患者外周血单个核细胞(PBMC)的IFN-γ的产生以及对不同T淋巴细胞亚群的影响,为TP5临床治疗重症肌无力提供实验学依据.方法重症肌无力患者PBMC在抗CD3单克隆抗体的刺激下,观察TP5对IFN-γ产生的影响.同时使用流式细胞仪,在单个细胞水平上评价TP5对各亚群T淋巴细胞细胞因子IFN-γ表达的影响.结果用抗CD3单克隆抗体和TP5(300μg/ml)刺激后,所有重症肌无力患者PBMC的IFN-γ表达水平明显受抑制(P_(儿童)=0.0001,P_(成人)=0.01);正常对照儿童和成人PBMC的IFN-γ表达水平亦明显下降(P_(儿童)=0.009,P_(成人)=0.0001).同一年龄分层组的比较,TP5对PBMC的IFN-γ表达水平的抑制程度在儿童MG组中较正常对照组弱,统计学有差异,而成人MG组与对照组比较差异无统计学意义(P_(成人)=0.481);TP5明显抑制CD8+(P_(MG)=0.025,P_(正常)=0.002)和CD4+T(P_(MG)=0.009,P_(正常)=0.004)细胞IFN-γ表达.结论 TP5能抑制治疗重症肌无力患者的细胞免疫应答.
목적 탐토재불동자격조건하,흉선오태(TP5)대중증기무력환자외주혈단개핵세포(PBMC)적IFN-γ적산생이급대불동T림파세포아군적영향,위TP5림상치료중증기무력제공실험학의거.방법 중증기무력환자PBMC재항CD3단극륭항체적자격하,관찰TP5대IFN-γ산생적영향.동시사용류식세포의,재단개세포수평상평개TP5대각아군T림파세포세포인자IFN-γ표체적영향.결과 용항CD3단극륭항체화TP5(300μg/ml)자격후,소유중증기무력환자PBMC적IFN-γ표체수평명현수억제(P_(인동)=0.0001,P_(성인)=0.01);정상대조인동화성인PBMC적IFN-γ표체수평역명현하강(P_(인동)=0.009,P_(성인)=0.0001).동일년령분층조적비교,TP5대PBMC적IFN-γ표체수평적억제정도재인동MG조중교정상대조조약,통계학유차이,이성인MG조여대조조비교차이무통계학의의(P_(성인)=0.481);TP5명현억제CD8+(P_(MG)=0.025,P_(정상)=0.002)화CD4+T(P_(MG)=0.009,P_(정상)=0.004)세포IFN-γ표체.결론 TP5능억제치료중증기무력환자적세포면역응답.
Objective To evaluate the effects of TP5 upon the production of IFN-γ and different T cell subsets by human peripheral blood mononuclear cells (PBMCs) from patients with myasthenia gravis (MG) and to provide experimental rationales for TP5 in clinical therapy of MG. Methods PBMCs were isolated from peripheral blood of MG individuals and cultured with anti-CD3. The level of IFN-γ in culture supernatants was examined by ELISA. The subsets and frequency of IFN-γ-producing cells were examined at a single-cell level by flow cytometry. Results After PBMCs stimulation with anti-CD3 and TP5 (300 μg/ml) , the level of IFN-γ expression was significantly inhibited ( P_(child) =0. 0001 , P_(adult) =0. 01 ) ; and the level of IFN-γ expression from normal adult and child controls was also significantly inhibited (P_(child) = 0. 009,P_(adult) =0. 0001 ). In addition, the inhibition of TP5 on the production of IFN-γ by PBMCs from MG children was lower compared with normal child control. But as compared with normal adult control, the inhibition of TP5 showed no significant difference in MG adults ( P_(adult) = 0.481 ). TP5 inhibited the expression of IFN-γ by CD8 + T cell and CD4 + T cell. Conclusion TP5 can inhibit the response of cellular immune by decreasing the production of IFN-γ in MG consequence display that the level of IFN-γ significant decreased with the addition of TP5 and anti-CD3. But after considering the age, the level of IFN-γ in MG children was no as much inhibited as normal child. TP5 inhibits the expression of IFN-γ by CD8 + T and CD4 + T cells.