CAJ | 학술논문

被子植物在黑暗中萌发生长不能合成叶绿素和建成光合系统,但是将莲(Nelumbo nucife ra Gaertn.)胚芽置于黑暗中萌发生长时,却可以清楚地观察到它的光合系统进行发育: 首先,在原位低温荧光光谱上,LHCⅡ的荧光发射逐步红移变成典型的PSⅡ荧光发射,同时随着萌发时间的延长,PSⅠ的荧光发射也从无到有,逐渐增强;其次,对暗萌发10 d莲苗的叶绿体进行部分变性凝胶电泳分析也得到了PSⅠ的叶绿素蛋白复合物条带,通过Western blot s的蛋白免疫检测,在暗萌发莲苗中也证实了LHCⅠ组分中有Lhca1的存在;最后,对暗萌发莲苗叶绿体的PSⅡ和PSⅠ电子传递活性测量结果表明,在暗中发育形成的PSⅡ和PSⅠ核心都是有光化学活性的.文章讨论了莲胚芽暗萌发过程中进行光合系统发育的原因.
피자식물재흑암중맹발생장불능합성협록소화건성광합계통,단시장련(Nelumbo nucife ra Gaertn.)배아치우흑암중맹발생장시,각가이청초지관찰도타적광합계통진행발육: 수선,재원위저온형광광보상,LHCⅡ적형광발사축보홍이변성전형적PSⅡ형광발사,동시수착맹발시간적연장,PSⅠ적형광발사야종무도유,축점증강;기차,대암맹발10 d련묘적협록체진행부분변성응효전영분석야득도료PSⅠ적협록소단백복합물조대,통과Western blot s적단백면역검측,재암맹발련묘중야증실료LHCⅠ조분중유Lhca1적존재;최후,대암맹발련묘협록체적PSⅡ화PSⅠ전자전체활성측량결과표명,재암중발육형성적PSⅡ화PSⅠ핵심도시유광화학활성적.문장토론료련배아암맹발과정중진행광합계통발육적원인.
It is well known that no chlorophyll synthesis and photosystem biogenesis have b een detected in dark-grown angiosperm seedlings. However, in this report, we sho wed that both PSⅡ and PSⅠ could be formed in dark-grown lotus (Nelumbo nuci fera Gaertn.) seedlings. Lots of evidence were given: First, during the dark- grown peri od, the single fluorescence emission peak at 679 nm in lotus embryo red-shifted and transformed into the normal PSⅡ fluorescence emission; Simultaneously, PSⅠ f luorescence emission at 730 nm appeared and increased obviously; Second, with pa rtial denaturing SDS-PAGE method, PSⅠ chlorophyll-protein complex could be clea r ly separated from 10 days dark-grown lotus seedlings; Third, the existence of Lh ca1 was also proved by Western blots. Moreover, measurements of electron transfe r rate demonstrated that both PSⅡ and PSⅠ core in dark-grown lotus seedlin gs were photochemically active.