CAJ | 학술논문

目的观察大鼠帕金森综合征模型脑内黑质、尾状核及中缝核神经元超微结构变化,为阐述该病的发病机制及筛选有效药物,提供实验依据.方法选用健康成年 Wistar大鼠 12只,雌雄不限,按随机法选 9只为实验组 (注射盐酸哌替啶 ), 3只为正常对照组 (注射等量生理盐水 ).应用透射电镜观察经美兰块染的中脑黑质、脑桥中缝部及尾状核部.结果上述核团神经元的超微结构均发生病理性改变,神经细胞核膜皱缩,核膜凹凸不整,并有局部断裂;线粒体变性,基质浓度降低及空泡化;粗面内质网和高尔基复合体囊腔扩张变性;大量初级溶酶体及脂褐素集聚;出现了髓样体和多泡体等变性结构.结论黑质、尾状核及中缝核神经元超微结构的病理性变化从而导致纹状体-黑质-纹状体锥体外路系环路功能障碍,是引发帕金森综合征的结构基础.
목적관찰대서파금삼종합정모형뇌내흑질、미상핵급중봉핵신경원초미결구변화,위천술해병적발병궤제급사선유효약물,제공실험의거.방법선용건강성년 Wistar대서 12지,자웅불한,안수궤법선 9지위실험조 (주사염산고체정 ), 3지위정상대조조 (주사등량생리염수 ).응용투사전경관찰경미란괴염적중뇌흑질、뇌교중봉부급미상핵부.결과상술핵단신경원적초미결구균발생병이성개변,신경세포핵막추축,핵막요철불정,병유국부단렬;선립체변성,기질농도강저급공포화;조면내질망화고이기복합체낭강확장변성;대량초급용매체급지갈소집취;출현료수양체화다포체등변성결구.결론흑질、미상핵급중봉핵신경원초미결구적병이성변화종이도치문상체-흑질-문상체추체외로계배로공능장애,시인발파금삼종합정적결구기출.
Aim To observe the ultrastructure of substantia nigra,caudate nucleus and raphe nucleus in rat of parkinsonism model and provide experimental basis for etiology and screening of effective drugs.Methods 12 healthy adult Wistar rats were selected without limitation of sex and divided by random drawing lots into experiment group(pethidine hydrochloride was injected,n=9) and control group(normal saline was injected,n=3).Electron microscope was used to observe substantia nigra,caudate nucleus and raphe nucleus stained by methylene blue.Results The pathological changes in the nuclei mentioned above included the crimple and ruture of nucleus membrane,mitochondria degeneration and other degenerative structure such as plenty of primary lysosomes,agglomeration of lipochrome,Golgi complex degeneration and so on.Conclusion The pathological changes of substantia nigra,caudate nucleus and raphe nucleus lead to dysfunction of striatum-substantia nigra-striatum circle.It is the structural basis for the Parkinson's syndrome.