国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
7期
675-676,679
,共3页
卢业成%郑师陵%肖艳华%陈星%初德强
盧業成%鄭師陵%肖豔華%陳星%初德彊
로업성%정사릉%초염화%진성%초덕강
毛细管区带电泳%血红蛋白%血红蛋白病%地中海贫血
毛細管區帶電泳%血紅蛋白%血紅蛋白病%地中海貧血
모세관구대전영%혈홍단백%혈홍단백병%지중해빈혈
Capillary Zone Electrophoresis%Hemoglobin%Hemoglobinopathies%Thalassemia
目的 探讨全自动多通道毛细管区带电泳技术在血红蛋白分析中的临床应用价值.方法 采用法国Sebia Capillarys 2电泳系统(Version 5.50)及配套试剂,全血样品经系统自动处理后,在9.8 kV电压、34℃、pH9.4的缓冲液条件下,在8条并联的毛细管(17.5 cm×25μm)内进行血红蛋白电泳,415 nm下直接检测各组分的百分比浓度.结果 分析183例健康成人血红蛋白电泳结果 ,确定本实验室参考范围HbA2:2.1%～3.2%.应用Hb AFSC质控进行精密度试验,各组分的CV值在(批内:0.21%～1.07%;批间:0.60%～3.10%)之间.检测6 045例临床需要进行溶血性黄疸诊断和鉴别诊断的患者标本,检出HbA2增高379例;HbF增高203例;HbH带27例;HbE带8例;HbS带4例;Hb J-K带3例;Hb Bart's 2例;HbD 1例.结论 Sebia Capillarys 2电泳系统能够清晰分辨HbA2、HbF增高及其他异常区带;可准确地将HbC和HbE与HbA2区分;还可完美区分和聚焦HbA与HbS之间的HbF;并可以轻易区分移动速度相近的HbS和HbD.具有自动化程度高、操作简便、快速准确的特点,适合医疗机构临床实验室在血红蛋白分析中常规应用.
目的 探討全自動多通道毛細管區帶電泳技術在血紅蛋白分析中的臨床應用價值.方法 採用法國Sebia Capillarys 2電泳繫統(Version 5.50)及配套試劑,全血樣品經繫統自動處理後,在9.8 kV電壓、34℃、pH9.4的緩遲液條件下,在8條併聯的毛細管(17.5 cm×25μm)內進行血紅蛋白電泳,415 nm下直接檢測各組分的百分比濃度.結果 分析183例健康成人血紅蛋白電泳結果 ,確定本實驗室參攷範圍HbA2:2.1%～3.2%.應用Hb AFSC質控進行精密度試驗,各組分的CV值在(批內:0.21%～1.07%;批間:0.60%～3.10%)之間.檢測6 045例臨床需要進行溶血性黃疸診斷和鑒彆診斷的患者標本,檢齣HbA2增高379例;HbF增高203例;HbH帶27例;HbE帶8例;HbS帶4例;Hb J-K帶3例;Hb Bart's 2例;HbD 1例.結論 Sebia Capillarys 2電泳繫統能夠清晰分辨HbA2、HbF增高及其他異常區帶;可準確地將HbC和HbE與HbA2區分;還可完美區分和聚焦HbA與HbS之間的HbF;併可以輕易區分移動速度相近的HbS和HbD.具有自動化程度高、操作簡便、快速準確的特點,適閤醫療機構臨床實驗室在血紅蛋白分析中常規應用.
목적 탐토전자동다통도모세관구대전영기술재혈홍단백분석중적림상응용개치.방법 채용법국Sebia Capillarys 2전영계통(Version 5.50)급배투시제,전혈양품경계통자동처리후,재9.8 kV전압、34℃、pH9.4적완충액조건하,재8조병련적모세관(17.5 cm×25μm)내진행혈홍단백전영,415 nm하직접검측각조분적백분비농도.결과 분석183례건강성인혈홍단백전영결과 ,학정본실험실삼고범위HbA2:2.1%～3.2%.응용Hb AFSC질공진행정밀도시험,각조분적CV치재(비내:0.21%～1.07%;비간:0.60%～3.10%)지간.검측6 045례림상수요진행용혈성황달진단화감별진단적환자표본,검출HbA2증고379례;HbF증고203례;HbH대27례;HbE대8례;HbS대4례;Hb J-K대3례;Hb Bart's 2례;HbD 1례.결론 Sebia Capillarys 2전영계통능구청석분변HbA2、HbF증고급기타이상구대;가준학지장HbC화HbE여HbA2구분;환가완미구분화취초HbA여HbS지간적HbF;병가이경역구분이동속도상근적HbS화HbD.구유자동화정도고、조작간편、쾌속준학적특점,괄합의료궤구림상실험실재혈홍단백분석중상규응용.
Objective To explore the applicable value of automated capillary zone electrophoresis in hemoglobin analysis. Methods By using French Sebia Capillarys 2 electrophoresis system (Version 5.50) and related reagents, the whole blood samples were treated by the system automatically,and under the condition of 9.8 kV, 34 ℃, pH9.4 of buffer solution, hemoglobin electrophoresis was carried out within eight parallel capillaries (17.5 cm× 25 μm) and the percentage concentration of each component were directly detected at 415 nm. Results By analyzing the results of hemoglobin electrophoresis of 183 healthy adults, HbA2 reference range of our laboratory was established: 2.1%-3.2%.Precision test was performed by quality control of Hb AFSC, and CVs about each component ranged from (intra-assay: 0. 21% to 1.07%; inter-assay: 0.60% to 3.10%). In 6 045 samples which needed diagnosis and differential diagnosis of hemolytic jaundice clinically, 379 cases were detected with higher HbA2, 203 cases were detected with higher HbF, 27 cases were with HbH band, 8 cases with HbE band, 4 cases with HbS band, 3 cases with Hb J-K band, 2 cases with Hb Bart's band, and 1case was with HbD band. Conclusion Sebia Capillarys 2 electrophoresis system can clearly distinguish higher HbA2, higher HbF and other abnormal bands; can accurately distinguish HbC from HbE and HbA2 ; can also perfectly distinguish and focalize on HbF between HbA and HbS; and can easily distinguish HbS and HbD which have the close migration speed. With a high degree of automation, easy operation, rapid and accurate characteristics, it seems appropriate for conventional application of hemoglobin analysis in clinical laboratories of medical institutions.
