CAJ | 학술논문

目的观察Ad-GFP-nm23-Hl抑制人高转移肺巨细胞癌株95D细胞生长和转移的作用,为后期nm23-Hl基因和腺病毒载体用于95D及其他肿瘤的基因治疗提供一定的理论和方法.方法应用MTT法及Transwell小室法分别检测95D细胞经Ad-GFP-nm23-Hl作用后的细胞体外增殖活性、黏附能力以及侵袭力的变化.同时应用人高转移肺巨细胞癌株95D裸鼠移植瘤模型,研究Ad-GFP-nm23-Hl对此移植瘤生长的抑制作用.结果 10~8PFU/ml、10~9PFU/ml、10~(10)PFU/ml处理组,可以明显抑制95D细胞的增殖、黏附和侵袭能力,其抑制作用呈剂量-效应关系.10~9PFU/ml 的Ad-GFP-nm23-Hl对移植瘤的抑瘤率为38.23%,较对照组差异显著.结论 Ad-GFP-nm23-Hl对人高转移肺巨细胞癌株95D细胞的生长和转移以及95D实体瘤具有抑制作用.
목적 관찰Ad-GFP-nm23-Hl억제인고전이폐거세포암주95D세포생장화전이적작용,위후기nm23-Hl기인화선병독재체용우95D급기타종류적기인치료제공일정적이론화방법.방법 응용MTT법급Transwell소실법분별검측95D세포경Ad-GFP-nm23-Hl작용후적세포체외증식활성、점부능력이급침습력적변화.동시응용인고전이폐거세포암주95D라서이식류모형,연구Ad-GFP-nm23-Hl대차이식류생장적억제작용.결과 10~8PFU/ml、10~9PFU/ml、10~(10)PFU/ml처리조,가이명현억제95D세포적증식、점부화침습능력,기억제작용정제량-효응관계.10~9PFU/ml 적Ad-GFP-nm23-Hl대이식류적억류솔위38.23%,교대조조차이현저.결론 Ad-GFP-nm23-Hl대인고전이폐거세포암주95D세포적생장화전이이급95D실체류구유억제작용.
Objective to study the inhibitory effects of Ad-GFP-nm23-Hl on proliferation and metastasis of human pulmonary giant cell carcinoma cell line 95D, thus to provide theory and method basis for gene therapy of 95D and other cancers by nm23-Hl and adenoviral vectors in the later therapy. Methods MTT assay and transwell chambers were used to detect the rates of proliferation and invasion as well as the adhesion of human pulmonary giant cell carcinoma cell line 95D in vitro, meanwhile, the inhibitory effects of Ad-GFP-nm23-Hl on growth of human pulmonary giant cell carcinoma cancer xenografts in nude mice was investigated. Results Ad-GFP-nm23-Hl significantly inhibited the proliferation, adhesion and invasiveness of 95D when treated with PBS and Ad-GFP-nm23- Hl of 10~(10) PFU/ml, 10~9 PFU/ml and 10~8 PFU/ml. Solid tumors formed on nude mice were significantly inhibited by the 10~9 PFU/ml group as compared with the control group. The growth inhibitory rate was 38.23%. Conclusions Ad-GFP-nm23-Hl showed inhibitory effects on the proliferation and metastasis of human pulmonary giant cell carcinoma 95D as well as solid tumors formed on nude mice.