草业科学
草業科學
초업과학
PRATACULTURAL SCIENCE
2010年
3期
107-111
,共5页
日本结缕草%愈伤诱导%植株再生
日本結縷草%愈傷誘導%植株再生
일본결루초%유상유도%식주재생
zoysiagrass (Zoysia japonica Stend)%callus induction%plant regeneration
以日本结缕草Zoysia japonica Steud的成熟种子为外植体进行了胚性愈伤的诱导与植株再生,结果表明:种子经去皮预处理后, 含2.0 mg/L 2,4-D的MS基本培养基能成功诱导去皮种子产生愈伤组织,愈伤组织诱导率高达87.1%.愈伤组织在继代过程中共产生4种不同的类型.1/2 MS中添加0.4 mg/L KT或添加1.0 mg/L KT和1.0 mg/L NAA均能有效促进胚性愈伤的分化,分化率分别为63.8%、66.0%.
以日本結縷草Zoysia japonica Steud的成熟種子為外植體進行瞭胚性愈傷的誘導與植株再生,結果錶明:種子經去皮預處理後, 含2.0 mg/L 2,4-D的MS基本培養基能成功誘導去皮種子產生愈傷組織,愈傷組織誘導率高達87.1%.愈傷組織在繼代過程中共產生4種不同的類型.1/2 MS中添加0.4 mg/L KT或添加1.0 mg/L KT和1.0 mg/L NAA均能有效促進胚性愈傷的分化,分化率分彆為63.8%、66.0%.
이일본결루초Zoysia japonica Steud적성숙충자위외식체진행료배성유상적유도여식주재생,결과표명:충자경거피예처리후, 함2.0 mg/L 2,4-D적MS기본배양기능성공유도거피충자산생유상조직,유상조직유도솔고체87.1%.유상조직재계대과정중공산생4충불동적류형.1/2 MS중첨가0.4 mg/L KT혹첨가1.0 mg/L KT화1.0 mg/L NAA균능유효촉진배성유상적분화,분화솔분별위63.8%、66.0%.
In order to achieve embryogenic callus induction and plant regeneration, the sterilized mature seeds of zoysiagrass (Zoysia japonica Stend.) were used as explants. The results indicated that peeled seeds after pretreatment in MS containing 2.0 mg/L 2,4-D could successfully induce callus, the frequency was as high as 87.1%. In the course of subculture, there were four different types of callus. 1/2 MS containing 0.4 mg/L KT, or 1/2 MS containing 1.0 mg/L KT and 1.0 mg/L NAA was able to promote the differentiation of embryogenic callus effectively, the differentiation rate was 63.8% and 66.0%, respectively.
