林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2010年
1期
147-151
,共5页
乌云塔娜%李洪果%李振国%包梅荣%谭晓风
烏雲塔娜%李洪果%李振國%包梅榮%譚曉風
오운탑나%리홍과%리진국%포매영%담효풍
梨属%自交不亲和性%SFBB~(-γ)基因
梨屬%自交不親和性%SFBB~(-γ)基因
리속%자교불친화성%SFBB~(-γ)기인
Pyrus%self-incompatibility%SFBB~(-γ)gene
By using PCR-based molecular method,four new pear SFBB~(-γ) genes were isolated from 8 pear cultivars known as S-genotypes.Length of PCR products from eight pear cultivars was around 1 200 bp.Sequencing the specific PCR fragments revealed four new SFBB~(-γ) genes that were respectively named as SFBB~(16-γ) (EU422956),SFBB~(17-γ)(EU422957),SFBB~(28-γ)(EU422960) and SFBB~(35-γ)(EU422958).As for different SFBB~(-γ) genes,variation in amino acid was higher in Fbox region and variable region 1,and lower in variable region 2 to 4.Phylogenetic analysis showed that there was a high level of difference between Japanese pear (Pyrus pyrifolia) SFBB~(-γ) genes and Xinjiang pear(P.sinkiangensis) SFBB~(-γ) genes,as well as Japanese pear SFBB~(-γ) genes and Chinese white pear(P,bretschneideri) SFBB~(-γ) genes.The results might reflect their genetic relationships among the species.
