CAJ | 학술논문

本实验建立沙丁胺醇的小鼠病理模型,对其肝脏和心脏做组织冰冻切片HE染色后观察发现其肝脏损伤严重,肝小叶的肝细胞已不再以中央静脉成放射状排布,分布较散,且在肝细胞中有凋亡小体出现;心脏组织中大量的心肌纤维凝结出现凝同性状坏死现象.以病理小鼠的肝脏作为阳性样品用液相色谱-串联质谱法检测,对其肝脏的酶解必要性进行研究,测量结果显示,β-葡萄糖醛苷酶/芳基硫酸酯酶是沙丁胺醇检测的有效酶,经其酶解后检测的数值约为末经酶解的3倍.
본실험건립사정알순적소서병리모형,대기간장화심장주조직빙동절편HE염색후관찰발현기간장손상엄중,간소협적간세포이불재이중앙정맥성방사상배포,분포교산,차재간세포중유조망소체출현;심장조직중대량적심기섬유응결출현응동성상배사현상.이병리소서적간장작위양성양품용액상색보-천련질보법검측,대기간장적매해필요성진행연구,측량결과현시,β-포도당철감매/방기류산지매시사정알순검측적유효매,경기매해후검측적수치약위말경매해적3배.
A pathological mouse model induced by salbutamol wag established for evaluating its pathological roles.HE staining of frozen sections showed serious damages in liver tissues,which exhibited scattered distribution rather than radial-arranged central veins in liver leaflets and obvious apoptotic bodies in liver tissues.Simultaneously,coagulation necrosis wag also observed in heart tissues due to the coagulation of a large number of myocardial fibers.Moreover,the liver of pathological mice was used as a positive sample to detect its enzymatic hydrolysis using LC-MS/MS.Results indicated that β-D-glucuronic glucosidase or arylsulfatase was the effective enzyme for salbutamol determination and the determination results obtained using enzymatic hydrolysis was three times as high as those obtained without using enzymatic hydrolysis.