CAJ | 학술논문

猪生长激素基因(pGH)是猪生长发育性状重要的候选基因之一.采用PCR-RFLP技术检测藏猪pGH基因+159～+734 bp片段的Dra I、Apa I和Msp I酶切多态性.结果显示,藏猪群体中Dra I酶切全部切开,无多态;Apa I酶切具有4个等位基因,出现5种基因型,其中CC基因型(299A/432C)频率最高,为0.7857;藏猪群体Msp I酶切出现3种基因型,杂合性CT基因型频率最高,为0.5089,等位基因C和T频率分别为0.5580和0.4420.经比较,藏猪pGH基因Dra I酶切位点与其他猪种一样无多态性,而Apa I酶切出现了特异的432突变位点,Msp I酶切等位基因分布相对均衡,表现出与其他猪种的差异.
저생장격소기인(pGH)시저생장발육성상중요적후선기인지일.채용PCR-RFLP기술검측장저pGH기인+159～+734 bp편단적Dra I、Apa I화Msp I매절다태성.결과현시,장저군체중Dra I매절전부절개,무다태;Apa I매절구유4개등위기인,출현5충기인형,기중CC기인형(299A/432C)빈솔최고,위0.7857;장저군체Msp I매절출현3충기인형,잡합성CT기인형빈솔최고,위0.5089,등위기인C화T빈솔분별위0.5580화0.4420.경비교,장저pGH기인Dra I매절위점여기타저충일양무다태성,이Apa I매절출현료특이적432돌변위점,Msp I매절등위기인분포상대균형,표현출여기타저충적차이.
The porcine growth hormone (pGH) gene is one of important candidate for development and growth trait. In the present study, the polymorphism of fragment +159～+734 of pGH gene was measured with the methods of PCR-RFLP by restriction endonuclease Dra I, Apa I and Msp I. The results showed that the Tibet pig has no diversity for PCR-Dra I-RFLP of pGH gene. There are four alleles and five genotypes for PCR-Apa I-RFLP of pGH gene, and the genotype CC (299A/432C) is predominant (0.7857). The Tibet pig population has three genotypes for PCR-Msp I-RFLP of pGH gene, and the predominant genotype CT is (0.5089). The frequencies of allele C and allele T are 0.5580 and 0.4420 respectively. By comparing with other breeds, the Tibet pig has differential genotypes for Apa I, and the distribution of alleles for Msp I is uniform relatively, which may be the difference of Tibet pig with other pig breeds.