CAJ | 학술논문

目的:探讨一次大强度运动对骨骼肌收缩蛋白降解和26S蛋白酶体活性的影响.方法:36只雄性Sprague-Dawley大鼠随机分为6组:安静对照组、运动0.5小时组、运动1小时组、运动1小时后1小时组、运动1小时后2小时组、运动1小时后6小时组,每组6只.运动方式采用一次大强度跑台运动,速度为25m/min,坡度为5%,运动时间分别为0.5小时和1小时.运动后按每组规定时间取材大鼠腓肠肌,观察其3-甲基组氨基酸(3-MH)含量、26S蛋白酶体活性和26S蛋白酶体C2亚基mRNA表达的变化.结果:(1)运动(0.5小时和1小时)后即刻和运动1小时后6小时大鼠腓肠肌3-MH含量显著高于安静对照组(P<0.01),其中运动0.5小时即刻值最高.(2)运动0.5小时即刻、运动1小时后6小时大鼠腓肠肌26S蛋白酶体活性显著高于安静对照组(P<0.05).(3)运动1小时后6小时大鼠腓肠肌26S蛋白酶体C2亚基mRNA表达显著高于安静对照组(P<0.01),其它时间点却低于安静对照组.结论:运动后26S蛋白酶体活性增高可能促使骨骼肌收缩蛋白降解增强,其活性受亚基基因调控.
목적:탐토일차대강도운동대골격기수축단백강해화26S단백매체활성적영향.방법:36지웅성Sprague-Dawley대서수궤분위6조:안정대조조、운동0.5소시조、운동1소시조、운동1소시후1소시조、운동1소시후2소시조、운동1소시후6소시조,매조6지.운동방식채용일차대강도포태운동,속도위25m/min,파도위5%,운동시간분별위0.5소시화1소시.운동후안매조규정시간취재대서비장기,관찰기3-갑기조안기산(3-MH)함량、26S단백매체활성화26S단백매체C2아기mRNA표체적변화.결과:(1)운동(0.5소시화1소시)후즉각화운동1소시후6소시대서비장기3-MH함량현저고우안정대조조(P<0.01),기중운동0.5소시즉각치최고.(2)운동0.5소시즉각、운동1소시후6소시대서비장기26S단백매체활성현저고우안정대조조(P<0.05).(3)운동1소시후6소시대서비장기26S단백매체C2아기mRNA표체현저고우안정대조조(P<0.01),기타시간점각저우안정대조조.결론:운동후26S단백매체활성증고가능촉사골격기수축단백강해증강,기활성수아기기인조공.
Objective To explore the effects of an acute exercise on the contractile protein degradation and 26S proteasome activity in skeletal muscle.Methods Thirty-six male Sprague-Dawley rats were randomly divided into 6 groups,each group consisted of 6 rats.They were killed respectively at rest,immediately after half an hour of exercise(running on the treadmill at 25m/min,5% grade),and 1 hour,2h and 6h after one hour exercise.The 3-methylhistidine(3-MH)content,26S proteasome activity and C2 subunit mRNA expression in rat gastrocnemius muscle were detected.Resuits(1)3-MH content increased immediately and 6 hours after exercise,and reached its peak level immediately after half an hour of exercise(P<0.01).(2)26S proteasome activity increased immediately after half an hour of exercise and 6 hours after exercise(P<0.05).(3)Expression of 26S proteasome C2 subunit mRNA Was lower than baseline during and after exercise,but increased sharply 6 hours after exercise(P<0.01).Conclusion The increase in 26S proteasome activity regulated by subunits gene after exercise could enhance skeletal muscle contractile protein degradation